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Reduced Expression of the Vca0421 Gene of Vibrio Cholerae O1 Results in Innate Resistance to Ciprofloxacin

Overview
Journal Antimicrob Agents Chemother
Publisher American Society for Microbiology
Specialty Pharmacology
Date 2010 Sep 1
PMID 20805389
Citations 2
Authors
Jun Okuda
Naoki Hayashi
Yuka Wakahara
Naomasa Gotoh
Affiliations
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Abstract

A mini-Tn5 insertion into a ciprofloxacin (CIP)-resistant mutant of Vibrio cholerae O1 revealed that overexpression of the vca0421 gene, which encodes a hypothetical protein, in the CIP-resistant mutant carrying a mutation in the quinolone resistance-determining region (QRDR) of the gyrA gene causes sensitization to CIP. We propose a new intrinsic mechanism of resistance to fluoroquinolones due to the inherently reduced expression of the vca0421 gene in V. cholerae O1.

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References
1.
Baranwal S, Dey K, Ramamurthy T, Nair G, Kundu M . Role of active efflux in association with target gene mutations in fluoroquinolone resistance in clinical isolates of Vibrio cholerae. Antimicrob Agents Chemother. 2002; 46(8):2676-8. PMC: 127385. DOI: 10.1128/AAC.46.8.2676-2678.2002. View
2.
Okuda J, Nishibuchi M . Manifestation of the Kanagawa phenomenon, the virulence-associated phenotype, of Vibrio parahaemolyticus depends on a particular single base change in the promoter of the thermostable direct haemolysin gene. Mol Microbiol. 1998; 30(3):499-511. DOI: 10.1046/j.1365-2958.1998.01072.x. View
3.
Adachi T, Mizuuchi M, Robinson E, Appella E, ODea M, Gellert M . DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy. Nucleic Acids Res. 1987; 15(2):771-84. PMC: 340466. DOI: 10.1093/nar/15.2.771. View
4.
Okuda J, Ramamurthy T, Yamasaki S . Antibacterial activity of ciprofloxacin against clinical strains of Vibrio cholerae O139 recently isolated from India. Yakugaku Zasshi. 2007; 127(5):903-4. DOI: 10.1248/yakushi.127.903. View
5.
Peng H, Marians K . Escherichia coli topoisomerase IV. Purification, characterization, subunit structure, and subunit interactions. J Biol Chem. 1993; 268(32):24481-90. View