Zyxin-mediated Actin Assembly is Required for Efficient Wound Closure

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2010 Aug 31

PMID 20801875

Citations 24

Authors

Thuc Nghi Nguyen

Arisa Uemura

Wenting Shih

Soichiro Yamada

Affiliations

Soon will be listed here.

Abstract

Cytoskeletal regulation of cell adhesion is vital to the organization of multicellular structures. The focal adhesion protein zyxin emerged as a key regulator of actin assembly because zyxin recruits Enabled/vasodilator-stimulated phospho-proteins (Ena/VASP) to promote actin assembly. Zyxin also localizes to the sites of cell-cell adhesion and is thought to promote actin assembly with Ena/VASP. Using shRNA targeted to zyxin, we analyzed the roles of zyxin at adhesive contacts. In zyxin-deficient cells, the actin assembly at both focal adhesion and cell-cell adhesion was limited, but their migration rate was unchanged. Cell spreading on E-cadherin-coated surfaces and the formation of cell clusters were slower for zyxin-deficient cells than wild type cells. By ablating a single cell within a cell monolayer, we quantified the rate of wound closure driven by a contractile circumferential actin ring. Zyxin-deficient cells failed to recruit VASP to cell-cell junctions at the wound edge and had a slower wound closure rate than wild type cells. Our results suggest that, by recruiting VASP, zyxin regulates actin assembly at the sites of force-bearing cell-cell adhesion.

Citing Articles

Exploring Co-expression Modules-Traits Correlation through Weighted Gene Co-expression Network Analysis: A Promising Approach in Wound Healing Research.

Farhangniya M, Samadikuchaksaraei A, Mohamadi Farsani F Med J Islam Repub Iran. 2024; 38:82.

PMID: 39678778 PMC: 11644100. DOI: 10.47176/mjiri.38.82.

From stress fiber to focal adhesion: a role of actin crosslinkers in force transmission.

Katsuta H, Sokabe M, Hirata H Front Cell Dev Biol. 2024; 12:1444827.

PMID: 39193363 PMC: 11347286. DOI: 10.3389/fcell.2024.1444827.

The role of zyxin in signal transduction and its relationship with diseases.

Wu Z, Wu D, Zhong Q, Zou X, Liu Z, Long H Front Mol Biosci. 2024; 11:1371549.

PMID: 38712343 PMC: 11070705. DOI: 10.3389/fmolb.2024.1371549.

Zyxin is important for the stability and function of podocytes, especially during mechanical stretch.

Kliewe F, Siegerist F, Hammer E, Al-Hasani J, Amling T, Hollemann J Commun Biol. 2024; 7(1):446.

PMID: 38605154 PMC: 11009394. DOI: 10.1038/s42003-024-06125-5.

Spatial proximity of proteins surrounding zyxin under force-bearing conditions.

Cheah J, Jacobs K, Lai T, Caballelo R, Yee J, Ueda S Mol Biol Cell. 2021; 32(13):1221-1228.

PMID: 33909446 PMC: 8351546. DOI: 10.1091/mbc.E19-10-0568.

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