In Vitro Testing for Anti-inflammatory Properties of Compounds Employing Peripheral Blood Mononuclear Cells Freshly Isolated from Healthy Donors

Overview

Journal Inflamm Res

Specialties Allergy & Immunology
Pathology

Date 2010 Aug 27

PMID 20740299

Citations 29

Authors

M Jenny

M Klieber

D Zaknun

S Schroecksnadel

K Kurz

M Ledochowski

H Schennach

Dietmar Fuchs

Affiliations

Soon will be listed here.

Abstract

Introduction: Inflammation is crucially involved in a variety of diseases like autoimmune syndromes, cardiovascular and neurodegenerative disorders, cancer, sepsis and allograft rejection.

Methods: Freshly isolated human peripheral blood mononuclear cells (PBMCs) are used as a screening assay for anti-inflammatory properties of compounds. Determinations of neopterin production by ELISA and of tryptophan degradation by HPLC are used as read-outs. Results are compared with further markers of immune response and oxidative stress.

Results: Phytohaemagglutinin induced significant tryptophan degradation and neopterin formation in PBMC, which correlated with IFN-γ, TNF-α, soluble cytokine receptors and isoprostane-8. Addition of vitamin C and E suppressed the responses dose-dependently.

Discussion: The determination of tryptophan degradation and neopterin production in PBMC reflects various pro- and anti-inflammatory cascades that are of relevance also in patients. It constitutes a robust and reliable approach to screen anti-inflammatory or immunosuppressive drugs and may improve throughput, speed and cost-effectiveness in drug discovery.

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