Rad9 is Required for B Cell Proliferation and Immunoglobulin Class Switch Recombination

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2010 Aug 24

PMID 20729201

Citations 13

Authors

Lili An

Yulan Wang

Yuheng Liu

Xiao Yang

ChunChun Liu

Zhishang Hu

Wei He

Wenxia Song

Haiying Hang

Affiliations

Soon will be listed here.

Abstract

B cell maturation and B cell-mediated antibody response require programmed DNA modifications such as the V(D)J recombination, the immunoglobulin (Ig) class switch recombination, and the somatic hypermutation to generate functional Igs. Many protein factors involved in DNA damage repair have been shown to be critical for the maturation and activation of B cells. Rad9 plays an important role in both DNA repair and cell cycle checkpoint control. However, its role in Ig generation has not been reported. In this study, we generated a conditional knock-out mouse line in which Rad9 is deleted specifically in B cells and investigated the function of Rad9 in B cells. The Rad9(-/-) B cells isolated from the conditional knock-out mice displayed impaired growth response and enhanced DNA lesions. Impaired Ig production in response to immunization in Rad9(-/-) mice was also detected. In addition, the Ig class switch recombination is deficient in Rad9(-/-) B cells. Taken together, Rad9 plays dual roles in generating functional antibodies and in maintaining the integrity of the whole genome in B cells.

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