Rad9a is Required for Spermatogonia Differentiation in Mice

Overview

Journal Oncotarget

Specialty Oncology

Date 2016 Nov 19

PMID 27861152

Citations 2

Authors

Lin Huang

Zhen-Bo Wang

Shu-Tao Qi

Xue-Shan Ma

Qiu-Xia Liang

Guo Lei

Tie-Gang Meng

Li-Feng Liang

Ye-Xin Xian

Yi Hou

Xiao-Fang Sun

Yong Zhao

Wei-Hua Wang

Qing-Yuan Sun

Affiliations

Soon will be listed here.

Abstract

Spermatogenesis in testes requires precise spermatogonia differentiation. Spermatocytes lacking the Rad9a gene are arrested in pachytene prophase, implying a possible role for RAD9A in spermatogonia differentiation. However, numerous RAD9A-positive pachytene spermatocytes are still observed in mouse testes following Rad9a excision using the Stra8-Cre system, and it is unclear whether Rad9a deletion in spermatogonia interrupts differentiation. Here, we generated a mouse model in which Rad9a was specifically deleted in spermatogonial stem cells (SSCs) using Cre recombinase expression driven by the germ cell-specific Vasa promoter. Adult Rad9a-null male mice were infertile as a result of completely blocked spermatogonia differentiation. No early spermatocytes were detected in mutant testicular cords of 9-day-old mice. Mutant spermatogonia were prone to apoptosis, although proliferation rates were unaffected. Rad9a deletion also resulted in malformation of seminiferous tubules, in which cells assembled irregularly into clusters, and malformation led to testicular cord disruption. Our findings suggest that Rad9a is indispensable for spermatogonia differentiation and testicular development in mice.

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