CD34 is Required for Infiltration of Eosinophils into the Colon and Pathology Associated with DSS-induced Ulcerative Colitis

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 2010 Aug 11

PMID 20696776

Citations 23

Authors

Steven Maltby

Carolin Wohlfarth

Matthew Gold

Lori Zbytnuik

Michael R Hughes

Kelly M McNagny

Affiliations

Soon will be listed here.

Abstract

Eosinophil migration into the gut and the release of granular mediators plays a critical role in the pathogenesis of inflammatory bowel diseases, including ulcerative colitis. We recently demonstrated that eosinophil migration into the lung requires cell surface expression of the sialomucin CD34 on mast cells and eosinophils in an asthma model. Based on these findings, we investigated a similar role for CD34 in the migration of eosinophils and other inflammatory cells into the colon as well as explored the effects of CD34 ablation on disease development in a dextran sulfate sodium-induced model of ulcerative colitis. Our findings demonstrate decreased disease severity in dextran sulfate sodium-treated Cd34(-/-) mice, as assessed by weight loss, diarrhea, bleeding, colon shortening and tissue pathology, compared with wild-type controls. CD34 was predominantly expressed on eosinophils within inflamed colon tissues, and Cd34(-/-) animals exhibited drastically reduced colon eosinophil infiltration. Using chimeric animals, we demonstrated that decreased disease pathology resulted from loss of CD34 from bone marrow-derived cells and that eosinophilia in Cd34(-/-)IL5(Tg) animals was sufficient to overcome protection from disease. In addition, we demonstrated a decrease in peripheral blood eosinophil numbers following dextran sulfate sodium treatment. These findings demonstrate that CD34 was expressed on colon-infiltrating eosinophils and played a role in eosinophil migration. Further, our findings suggest CD34 is required for efficient eosinophil migration, but not proliferation or expansion, in the development of ulcerative colitis.

Citing Articles

CD34 Protein: Its expression and function in inflammation.

Rodrigues C, Moga S, Singh B, Aulakh G Cell Tissue Res. 2023; 393(3):443-454.

PMID: 37450038 DOI: 10.1007/s00441-023-03811-4.

Role of CD34 in inflammatory bowel disease.

Li Z, Dong S, Huang S, Sun Y, Sun Y, Zhao B Front Physiol. 2023; 14:1144980.

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Potent CCR3 Receptor Antagonist, SB328437, Suppresses Colonic Eosinophil Chemotaxis and Inflammation in the Murine Model of Spontaneous Chronic Colitis.

Filippone R, Dargahi N, Eri R, Uranga J, Bornstein J, Apostolopoulos V Int J Mol Sci. 2022; 23(14).

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Transcriptional Profiling of Mouse Eosinophils Identifies Distinct Gene Signatures Following Cellular Activation.

Dolitzky A, Shapira G, Grisaru-Tal S, Hazut I, Avlas S, Gordon Y Front Immunol. 2021; 12:802839.

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Inhibition of Prolyl Oligopeptidase Prevents Consequences of Reperfusion following Intestinal Ischemia.

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