CRISPR/Cas Technologies and Their Applications in

Overview

Journal Front Bioeng Biotechnol

Date 2021 Dec 3

PMID 34858961

Citations 9

Authors

Huina Dong

Yali Cui

Dawei Zhang

Affiliations

Soon will be listed here.

Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have revolutionized genome editing and greatly promoted the development of biotechnology. However, these systems unfortunately have not been developed and applied in bacteria as extensively as in eukaryotic organism. Here, the research progress on the most widely used CRISPR/Cas tools and their applications in is summarized. Genome editing based on homologous recombination, non-homologous DNA end-joining, transposons, and base editors are discussed. Finally, the state of the art of transcriptional regulation using CRISPRi is briefly reviewed. This review provides a useful reference for the application of CRISPR/Cas systems in other bacterial species.

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