Induction of Pluripotent Stem Cells from Human Third Molar Mesenchymal Stromal Cells

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2010 Jul 3

PMID 20595386

Citations 49

Authors

Yasuaki Oda

Yasuhide Yoshimura

Hiroe Ohnishi

Mika Tadokoro

Yoshihiro Katsube

Mari Sasao

Yoko Kubo

Koji Hattori

Shigeru Saito

Katsuhisa Horimoto

Shunsuke Yuba

Hajime Ohgushi

Affiliations

Soon will be listed here.

Abstract

The expression of four transcription factors (OCT3/4, SOX2, KLF4, and MYC) can reprogram mouse as well as human somatic cells to induced pluripotent stem (iPS) cells. We generated iPS cells from mesenchymal stromal cells (MSCs) derived from human third molars (wisdom teeth) by retroviral transduction of OCT3/4, SOX2, and KLF4 without MYC, which is considered as oncogene. Interestingly, some of the clonally expanded MSCs could be used for iPS cell generation with 30-100-fold higher efficiency when compared with that of other clonally expanded MSCs and human dermal fibroblasts. Global gene expression profiles demonstrated some up-regulated genes regarding DNA repair/histone conformational change in the efficient clones, suggesting that the processes of chromatin remodeling have important roles in the cascade of iPS cells generation. The generated iPS cells resembled human embryonic stem (ES) cells in many aspects, including morphology, ES marker expression, global gene expression, epigenetic states, and the ability to differentiate into the three germ layers in vitro and in vivo. Because human third molars are discarded as clinical waste, our data indicate that clonally expanded MSCs derived from human third molars are a valuable cell source for the generation of iPS cells.

Citing Articles

A Brief History of Cell Culture: From Harrison to Organs-on-a-Chip.

Moro L, Guarnier L, Azevedo M, Fracasso J, Lucio M, Castro M Cells. 2025; 13(24.

PMID: 39768159 PMC: 11674496. DOI: 10.3390/cells13242068.

Advancements and Innovative Strategies in Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cell Therapy: A Comprehensive Review.

Shi X, Zhang K, Yu F, Qi Q, Cai X, Zhang Y Stem Cells Int. 2024; 2024:4073485.

PMID: 39377039 PMC: 11458320. DOI: 10.1155/2024/4073485.

From Pluripotent Stem Cells to Organoids and Bioprinting: Recent Advances in Dental Epithelium and Ameloblast Models to Study Tooth Biology and Regeneration.

Hermans F, Hasevoets S, Vankelecom H, Bronckaers A, Lambrichts I Stem Cell Rev Rep. 2024; 20(5):1184-1199.

PMID: 38498295 PMC: 11222197. DOI: 10.1007/s12015-024-10702-w.

Functional genomics in stem cell models: considerations and applications.

Shevade K, Peddada S, Mader K, Przybyla L Front Cell Dev Biol. 2023; 11:1236553.

PMID: 37554308 PMC: 10404852. DOI: 10.3389/fcell.2023.1236553.

Dental applications of induced pluripotent stem cells and their derivatives.

Gao P, Liu S, Wang X, Ikeya M Jpn Dent Sci Rev. 2022; 58:162-171.

PMID: 35516907 PMC: 9065891. DOI: 10.1016/j.jdsr.2022.03.002.

References

Cheadle C, Vawter M, Freed W, Becker K . Analysis of microarray data using Z score transformation. J Mol Diagn. 2003; 5(2):73-81. PMC: 1907322. DOI: 10.1016/S1525-1578(10)60455-2. View

Lilyestrom W, van der Woerd M, Clark N, Luger K . Structural and biophysical studies of human PARP-1 in complex with damaged DNA. J Mol Biol. 2009; 395(5):983-94. PMC: 2864582. DOI: 10.1016/j.jmb.2009.11.062. View

Kawasaki H, Mizuseki K, Nishikawa S, Kaneko S, Kuwana Y, Nakanishi S . Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity. Neuron. 2000; 28(1):31-40. DOI: 10.1016/s0896-6273(00)00083-0. View

Han J, Yuan P, Yang H, Zhang J, Soh B, Li P . Tbx3 improves the germ-line competency of induced pluripotent stem cells. Nature. 2010; 463(7284):1096-100. PMC: 2901797. DOI: 10.1038/nature08735. View

Rouleau M, Patel A, Hendzel M, Kaufmann S, Poirier G . PARP inhibition: PARP1 and beyond. Nat Rev Cancer. 2010; 10(4):293-301. PMC: 2910902. DOI: 10.1038/nrc2812. View

Viswanathan S, Powers J, Einhorn W, Hoshida Y, Ng T, Toffanin S . Lin28 promotes transformation and is associated with advanced human malignancies. Nat Genet. 2009; 41(7):843-8. PMC: 2757943. DOI: 10.1038/ng.392. View

Okita K, Ichisaka T, Yamanaka S . Generation of germline-competent induced pluripotent stem cells. Nature. 2007; 448(7151):313-7. DOI: 10.1038/nature05934. View

Zhao Y, Yin X, Qin H, Zhu F, Liu H, Yang W . Two supporting factors greatly improve the efficiency of human iPSC generation. Cell Stem Cell. 2008; 3(5):475-9. DOI: 10.1016/j.stem.2008.10.002. View

Hacein-Bey-Abina S, Garrigue A, Wang G, Soulier J, Lim A, Morillon E . Insertional oncogenesis in 4 patients after retrovirus-mediated gene therapy of SCID-X1. J Clin Invest. 2008; 118(9):3132-42. PMC: 2496963. DOI: 10.1172/JCI35700. View

10.

Cavazzana-Calvo M, Fischer A . Gene therapy for severe combined immunodeficiency: are we there yet?. J Clin Invest. 2007; 117(6):1456-65. PMC: 1878528. DOI: 10.1172/JCI30953. View

11.

Ebert A, Yu J, Rose Jr F, Mattis V, Lorson C, Thomson J . Induced pluripotent stem cells from a spinal muscular atrophy patient. Nature. 2008; 457(7227):277-80. PMC: 2659408. DOI: 10.1038/nature07677. View

12.

Pandita T, Richardson C . Chromatin remodeling finds its place in the DNA double-strand break response. Nucleic Acids Res. 2009; 37(5):1363-77. PMC: 2655678. DOI: 10.1093/nar/gkn1071. View

13.

Park I, Zhao R, West J, Yabuuchi A, Huo H, Ince T . Reprogramming of human somatic cells to pluripotency with defined factors. Nature. 2007; 451(7175):141-6. DOI: 10.1038/nature06534. View

14.

Takahashi K, Yamanaka S . Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-76. DOI: 10.1016/j.cell.2006.07.024. View

15.

Dimos J, Rodolfa K, Niakan K, Weisenthal L, Mitsumoto H, Chung W . Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons. Science. 2008; 321(5893):1218-21. DOI: 10.1126/science.1158799. View

16.

Maherali N, Sridharan R, Xie W, Utikal J, Eminli S, Arnold K . Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution. Cell Stem Cell. 2008; 1(1):55-70. DOI: 10.1016/j.stem.2007.05.014. View

17.

Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T . Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat Biotechnol. 2007; 26(1):101-6. DOI: 10.1038/nbt1374. View

18.

Wernig M, Meissner A, Foreman R, Brambrink T, Ku M, Hochedlinger K . In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature. 2007; 448(7151):318-24. DOI: 10.1038/nature05944. View

19.

Aasen T, Raya A, Barrero M, Garreta E, Consiglio A, Gonzalez F . Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol. 2008; 26(11):1276-84. DOI: 10.1038/nbt.1503. View

20.

van Attikum H, Gasser S . Crosstalk between histone modifications during the DNA damage response. Trends Cell Biol. 2009; 19(5):207-17. DOI: 10.1016/j.tcb.2009.03.001. View