Nanoparticle (NP) Uptake by Type I Alveolar Epithelial Cells and Their Oxidant Stress Response

Overview

Journal Nanotoxicology

Publisher Informa Healthcare

Specialties Biotechnology
Toxicology

Date 2011 Sep 28

PMID 20563262

Citations 21

Authors

Beth A Vanwinkle

Karen L de Mesy Bentley

Jonathan M Malecki

Karlene K Gunter

Irene M Evans

Alison Elder

Jacob N Finkelstein

Gunter Oberdorster

Thomas E Gunter

Affiliations

Soon will be listed here.

Abstract

Mammalian cells take up nanoparticles (NPs) and some NPs increase ROS. We use imaging and measure ROS in parallel to evaluate NP-cell interactions with type I-like alveolar epithelial cells exposed to NPs at 1.2 µg/cm(2) . Titanium dioxide (Ti0(2)), gold (Au), silver (Ag), and manganese (Mn) were internalized by R3-1 cells; copper (Cu) NPs were observed at the cell surface only. TiO(2) and Au did not increase cell death but Mn and Cu did, with surviving cells recovering after initial Cu exposure. Ag NPs caused 80% of R3-1 cells to lift off the slides within one hour. Amplex Red was used to report H(2)O(2) production after exposure to 0.4 µg/cm(2) TiO(2), Au, Cu, Mn and Ag. TiO(2), Au, and Ag caused no significant increase in H(2)O(2) while Cu and Mn increased H(2)O(2). NPs that give up electrons, increase ROS production and cause cell death in R3-1 cells.

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References

Li N, Sioutas C, Cho A, Schmitz D, Misra C, Sempf J . Ultrafine particulate pollutants induce oxidative stress and mitochondrial damage. Environ Health Perspect. 2003; 111(4):455-60. PMC: 1241427. DOI: 10.1289/ehp.6000. View

Wingrove D, Amatruda J, GUNTER T . Glucagon effects on the membrane potential and calcium uptake rate of rat liver mitochondria. J Biol Chem. 1984; 259(15):9390-4. View

Carlson C, Hussain S, Schrand A, Braydich-Stolle L, Hess K, Jones R . Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species. J Phys Chem B. 2008; 112(43):13608-19. DOI: 10.1021/jp712087m. View

Arbab A, Liu W, Frank J . Cellular magnetic resonance imaging: current status and future prospects. Expert Rev Med Devices. 2006; 3(4):427-39. DOI: 10.1586/17434440.3.4.427. View

Limbach L, Wick P, Manser P, Grass R, Bruinink A, Stark W . Exposure of engineered nanoparticles to human lung epithelial cells: influence of chemical composition and catalytic activity on oxidative stress. Environ Sci Technol. 2007; 41(11):4158-63. DOI: 10.1021/es062629t. View

Koslowski R, Barth K, Augstein A, Tschernig T, Bargsten G, Aufderheide M . A new rat type I-like alveolar epithelial cell line R3/1: bleomycin effects on caveolin expression. Histochem Cell Biol. 2004; 121(6):509-19. DOI: 10.1007/s00418-004-0662-4. View

Hussain S, Javorina A, Schrand A, Duhart H, Ali S, Schlager J . The interaction of manganese nanoparticles with PC-12 cells induces dopamine depletion. Toxicol Sci. 2006; 92(2):456-63. DOI: 10.1093/toxsci/kfl020. View

Brookes P, Yoon Y, Robotham J, Anders M, Sheu S . Calcium, ATP, and ROS: a mitochondrial love-hate triangle. Am J Physiol Cell Physiol. 2004; 287(4):C817-33. DOI: 10.1152/ajpcell.00139.2004. View

Paciotti G, Myer L, Weinreich D, Goia D, Pavel N, McLaughlin R . Colloidal gold: a novel nanoparticle vector for tumor directed drug delivery. Drug Deliv. 2004; 11(3):169-83. DOI: 10.1080/10717540490433895. View

10.

F Turrens J . Mitochondrial formation of reactive oxygen species. J Physiol. 2003; 552(Pt 2):335-44. PMC: 2343396. DOI: 10.1113/jphysiol.2003.049478. View

11.

Sheftel A, Zhang A, Brown C, Shirihai O, Ponka P . Direct interorganellar transfer of iron from endosome to mitochondrion. Blood. 2007; 110(1):125-32. DOI: 10.1182/blood-2007-01-068148. View

12.

Alt V, Bechert T, Steinrucke P, Wagener M, Seidel P, Dingeldein E . An in vitro assessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement. Biomaterials. 2004; 25(18):4383-91. DOI: 10.1016/j.biomaterials.2003.10.078. View

13.

Elder A, Oberdorster G . Translocation and effects of ultrafine particles outside of the lung. Clin Occup Environ Med. 2006; 5(4):785-96. DOI: 10.1016/j.coem.2006.07.003. View

14.

Kroemer G, Zamzami N, Susin S . Mitochondrial control of apoptosis. Immunol Today. 1997; 18(1):44-51. DOI: 10.1016/s0167-5699(97)80014-x. View

15.

Chen H, Detmer S, Ewald A, Griffin E, Fraser S, Chan D . Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J Cell Biol. 2003; 160(2):189-200. PMC: 2172648. DOI: 10.1083/jcb.200211046. View

16.

Shukla A, Gulumian M, Hei T, Kamp D, Rahman Q, Mossman B . Multiple roles of oxidants in the pathogenesis of asbestos-induced diseases. Free Radic Biol Med. 2003; 34(9):1117-29. DOI: 10.1016/s0891-5849(03)00060-1. View

17.

Gunter T, Gavin C, Aschner M, Gunter K . Speciation of manganese in cells and mitochondria: a search for the proximal cause of manganese neurotoxicity. Neurotoxicology. 2006; 27(5):765-76. DOI: 10.1016/j.neuro.2006.05.002. View

18.

Ji J, Jung J, Kim S, Yoon J, Park J, Choi B . Twenty-eight-day inhalation toxicity study of silver nanoparticles in Sprague-Dawley rats. Inhal Toxicol. 2007; 19(10):857-71. DOI: 10.1080/08958370701432108. View

19.

Bleazard W, McCaffery J, King E, Bale S, Mozdy A, Tieu Q . The dynamin-related GTPase Dnm1 regulates mitochondrial fission in yeast. Nat Cell Biol. 1999; 1(5):298-304. PMC: 3739991. DOI: 10.1038/13014. View

20.

Hainfeld J, Slatkin D, Focella T, Smilowitz H . Gold nanoparticles: a new X-ray contrast agent. Br J Radiol. 2006; 79(939):248-53. DOI: 10.1259/bjr/13169882. View