Beta-Endorphin Expression in the Mouse Retina

Overview

Journal J Comp Neurol

Specialty Neurology

Date 2010 Jun 10

PMID 20533364

Citations 17

Authors

Shannon K Gallagher

Paul Witkovsky

Michel J Roux

Malcolm J Low

Veronica Otero-Corchon

Shane T Hentges

Jozsef Vigh

Affiliations

Soon will be listed here.

Abstract

Evidence showing expression of endogenous opioids in the mammalian retina is sparse. In the present study we examined a transgenic mouse line expressing an obligate dimerized form of Discosoma red fluorescent protein (DsRed) under the control of the pro-opiomelanocortin promoter and distal upstream regulatory elements to assess whether pro-opiomelanocortin peptide (POMC), and its opioid cleavage product, beta-endorphin, are expressed in the mouse retina. Using double label immunohistochemistry we found that DsRed fluorescence was restricted to a subset of GAD-67-positive cholinergic amacrine cells of both orthotopic and displaced subtypes. About 50% of cholinergic amacrine cells colocalized DsRed and a large fraction of DsRed-expressing amacrine cells was positive for beta-endorphin immunostaining, whereas beta-endorphin-immunoreactive neurons were absent in retinas of POMC null mice. Our findings contribute to a growing body of evidence demonstrating that opioid peptides are an integral component of vertebrate retinas, including those of mammals.

Citing Articles

Dopamine enhances GABA receptor-mediated current amplitude in a subset of intrinsically photosensitive retinal ganglion cells.

Bergum N, Berezin C, Vigh J J Neurophysiol. 2024; 132(2):501-513.

PMID: 38958282 PMC: 11427049. DOI: 10.1152/jn.00457.2023.

Dopamine enhances GABA receptor-mediated current amplitude in a subset of intrinsically photosensitive retinal ganglion cells.

Bergum N, Berezin C, Vigh J bioRxiv. 2024; .

PMID: 38168350 PMC: 10760026. DOI: 10.1101/2023.12.11.571141.

Morphine pharmacokinetics and opioid transporter expression at the blood-retina barrier of male and female mice.

Berezin C, Bergum N, Torres Lopez G, Vigh J Front Pharmacol. 2023; 14:1206104.

PMID: 37388441 PMC: 10301758. DOI: 10.3389/fphar.2023.1206104.

Endogenous opioid signaling in the retina modulates sleep/wake activity in mice.

Berezin C, Bergum N, Luchini K, Curdts S, Korkis C, Vigh J Neurobiol Sleep Circadian Rhythms. 2022; 13:100078.

PMID: 35800978 PMC: 9254600. DOI: 10.1016/j.nbscr.2022.100078.

Trilogy Development of Proopiomelanocortin Neurons From Embryonic to Adult Stages in the Mice Retina.

Zhang X, Wang X, Wang S, Peng W, Ullah R, Fu J Front Cell Dev Biol. 2021; 9:718851.

PMID: 34676208 PMC: 8523858. DOI: 10.3389/fcell.2021.718851.

References

Yamamoto T, Yamato E, Tashiro F, Sato T, Noso S, Ikegami H . Development of autoimmune diabetes in glutamic acid decarboxylase 65 (GAD65) knockout NOD mice. Diabetologia. 2003; 47(2):221-4. DOI: 10.1007/s00125-003-1296-0. View

Husain S, Potter D, Crosson C . Opioid receptor-activation: retina protected from ischemic injury. Invest Ophthalmol Vis Sci. 2009; 50(8):3853-9. DOI: 10.1167/iovs.08-2907. View

Marc R, Murry R, Fisher S, Linberg K, Lewis G, Kalloniatis M . Amino acid signatures in the normal cat retina. Invest Ophthalmol Vis Sci. 1998; 39(9):1685-93. View

Kong J, Fish D, Rockhill R, Masland R . Diversity of ganglion cells in the mouse retina: unsupervised morphological classification and its limits. J Comp Neurol. 2005; 489(3):293-310. DOI: 10.1002/cne.20631. View

Raymond I, Vila A, Huynh U, Brecha N . Cyan fluorescent protein expression in ganglion and amacrine cells in a thy1-CFP transgenic mouse retina. Mol Vis. 2008; 14:1559-74. PMC: 2519030. View

Watt C, Li T, Lam D, Wu S . Quantitative studies of enkephalin's coexistence with gamma-aminobutyric acid, glycine and neurotensin in amacrine cells of the chicken retina. Brain Res. 1988; 444(2):366-70. DOI: 10.1016/0006-8993(88)90948-1. View

Voigt T . Cholinergic amacrine cells in the rat retina. J Comp Neurol. 1986; 248(1):19-35. DOI: 10.1002/cne.902480103. View

Zalutsky R, Miller R . The physiology of substance P in the rabbit retina. J Neurosci. 1990; 10(2):394-402. PMC: 6570149. View

Wassle H, Riemann H . The mosaic of nerve cells in the mammalian retina. Proc R Soc Lond B Biol Sci. 1978; 200(1141):441-61. DOI: 10.1098/rspb.1978.0026. View

10.

Whitney I, Keeley P, Raven M, Reese B . Spatial patterning of cholinergic amacrine cells in the mouse retina. J Comp Neurol. 2008; 508(1):1-12. PMC: 2414441. DOI: 10.1002/cne.21630. View

11.

Hokfelt T, Broberger C, Xu Z, Sergeyev V, Ubink R, Diez M . Neuropeptides--an overview. Neuropharmacology. 2000; 39(8):1337-56. DOI: 10.1016/s0028-3908(00)00010-1. View

12.

Gabriel R, Volgyi B, Pollak E . Calretinin-immunoreactive elements in the retina and optic tectum of the frog, Rana esculenta. Brain Res. 1998; 782(1-2):53-62. DOI: 10.1016/s0006-8993(97)01261-4. View

13.

Oliva Jr A, Jiang M, Lam T, Smith K, Swann J . Novel hippocampal interneuronal subtypes identified using transgenic mice that express green fluorescent protein in GABAergic interneurons. J Neurosci. 2000; 20(9):3354-68. PMC: 6773106. View

14.

Peng P, Huang H, Lee Y, Chen Y, Ma M . Novel role for the delta-opioid receptor in hypoxic preconditioning in rat retinas. J Neurochem. 2008; 108(3):741-54. DOI: 10.1111/j.1471-4159.2008.05807.x. View

15.

Vaney D, Whitington G, Young H . The morphology and topographic distribution of substance-P-like immunoreactive amacrine cells in the cat retina. Proc R Soc Lond B Biol Sci. 1989; 237(1289):471-88. DOI: 10.1098/rspb.1989.0060. View

16.

Chang Y, Gottlieb D . Characterization of the proteins purified with monoclonal antibodies to glutamic acid decarboxylase. J Neurosci. 1988; 8(6):2123-30. PMC: 6569335. View

17.

Choi J, Lee C, Yoo K, Hwang I, Lee I, Lee Y . Age-related changes in calbindin-D28k, parvalbumin, and calretinin immunoreactivity in the dog main olfactory bulb. Cell Mol Neurobiol. 2009; 30(1):1-12. PMC: 11498623. DOI: 10.1007/s10571-009-9425-z. View

18.

Brunelli G, Spano P, Barlati S, Guarneri B, Barbon A, Bresciani R . Glutamatergic reinnervation through peripheral nerve graft dictates assembly of glutamatergic synapses at rat skeletal muscle. Proc Natl Acad Sci U S A. 2005; 102(24):8752-7. PMC: 1142481. DOI: 10.1073/pnas.0500530102. View

19.

Tamamaki N, Yanagawa Y, Tomioka R, Miyazaki J, Obata K, Kaneko T . Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse. J Comp Neurol. 2003; 467(1):60-79. DOI: 10.1002/cne.10905. View

20.

Costa B, Hokoc J . Coexistence of GAD-65 and GAD-67 with tyrosine hydroxylase and nitric oxide synthase in amacrine and interplexiform cells of the primate, Cebus apella. Vis Neurosci. 2003; 20(2):153-63. DOI: 10.1017/s0952523803202066. View