Quantitative Studies of Enkephalin's Coexistence with Gamma-aminobutyric Acid, Glycine and Neurotensin in Amacrine Cells of the Chicken Retina

Previous double-label studies demonstrate that enkephalin coexists with gamma-aminobutyric acid, glycine or neurotensin in amacrine cells of the chicken retina. The present study utilizes double- and triple-label paradigms to quantitatively analyze these coexisting relationships. Twenty-eight percent of enkephalin-like immunoreactive amacrine cells were found to exhibit high-affinity uptake of [3H]GABA, while 53% of enkephalin-amacrine cells specifically accumulate [3H]glycine. Moreover, the present study predicts that at least 26% of enkephalin-amacrine cells which accumulate [3H]glycine should also be immunoreactive for neurotensin.