Comparative Study of the Antioxidant and Reactive Oxygen Species Scavenging Properties in the Extracts of the Fruits of Terminalia Chebula, Terminalia Belerica and Emblica Officinalis

Overview

Journal BMC Complement Altern Med

Publisher Biomed Central

Specialty Rehabilitation Medicine

Date 2010 May 14

PMID 20462461

Citations 59

Authors

Bibhabasu Hazra

Rhitajit Sarkar

Santanu Biswas

Nripendranath Mandal

Affiliations

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Abstract

Background: Cellular damage caused by reactive oxygen species (ROS) has been implicated in several diseases, and hence natural antioxidants have significant importance in human health. The present study was carried out to evaluate the in vitro antioxidant and reactive oxygen species scavenging activities of Terminalia chebula, Terminalia belerica and Emblica officinalis fruit extracts.

Methods: The 70% methanol extracts were studied for in vitro total antioxidant activity along with phenolic and flavonoid contents and reducing power. Scavenging ability of the extracts for radicals like DPPH, hydroxyl, superoxide, nitric oxide, hydrogen peroxide, peroxynitrite, singlet oxygen, hypochlorous acid were also performed to determine the potential of the extracts.

Results: The ability of the extracts of the fruits in exhibiting their antioxative properties follow the order T. chebula >E. officinalis >T. belerica. The same order is followed in their flavonoid content, whereas in case of phenolic content it becomes E. officinalis >T. belerica >T. chebula. In the studies of free radicals' scavenging, where the activities of the plant extracts were inversely proportional to their IC50 values, T. chebula and E. officinalis were found to be taking leading role with the orders of T. chebula >E. officinalis >T. belerica for superoxide and nitric oxide, and E. officinalis >T. belerica >T. chebula for DPPH and peroxynitrite radicals. Miscellaneous results were observed in the scavenging of other radicals by the plant extracts, viz., T. chebula >T. belerica >E. officinalis for hydroxyl, T. belerica >T. chebula >E. officinalis for singlet oxygen and T. belerica >E. officinalis >T. chebula for hypochlorous acid. In a whole, the studied fruit extracts showed quite good efficacy in their antioxidant and radical scavenging abilities, compared to the standards.

Conclusions: The evidences as can be concluded from the study of the 70% methanol extract of the fruits of Terminalia chebula, Terminalia belerica and Emblica officinalis, imposes the fact that they might be useful as potent sources of natural antioxidant.

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References

Huie R, Padmaja S . The reaction of no with superoxide. Free Radic Res Commun. 1993; 18(4):195-9. DOI: 10.3109/10715769309145868. View

Kochevar I, Redmond R . Photosensitized production of singlet oxygen. Methods Enzymol. 2000; 319:20-8. DOI: 10.1016/s0076-6879(00)19004-4. View

Ellman G . Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82(1):70-7. DOI: 10.1016/0003-9861(59)90090-6. View

Robak J, Gryglewski R . Flavonoids are scavengers of superoxide anions. Biochem Pharmacol. 1988; 37(5):837-41. DOI: 10.1016/0006-2952(88)90169-4. View

Finkel T, Holbrook N . Oxidants, oxidative stress and the biology of ageing. Nature. 2000; 408(6809):239-47. DOI: 10.1038/35041687. View

Gu X, Chen C, Thou T . Spectrophotometric method for the determination of ascorbic acid with iron (III)-1,10-phenanthroline after preconcentration on an organic solvent-soluble membrane filter. Anal Bioanal Chem. 1996; 355(1):94-5. DOI: 10.1007/s0021663550094. View

Yildirim A, Mavi A, Oktay M, Kara A, Algur O, Bilaloglu V . Comparison of antioxidant and antimicrobial activities of tilia (Tilia argentea Desf ex DC), sage (Salvia triloba l.), and black tea (Camellia sinensis) extracts. J Agric Food Chem. 2000; 48(10):5030-4. DOI: 10.1021/jf000590k. View

Beyer R . The role of ascorbate in antioxidant protection of biomembranes: interaction with vitamin E and coenzyme Q. J Bioenerg Biomembr. 1994; 26(4):349-58. DOI: 10.1007/BF00762775. View

Hazra B, Biswas S, Mandal N . Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complement Altern Med. 2008; 8:63. PMC: 2636748. DOI: 10.1186/1472-6882-8-63. View

10.

Cheng H, Lin T, Yu K, Yang C, Lin C . Antioxidant and free radical scavenging activities of Terminalia chebula. Biol Pharm Bull. 2003; 26(9):1331-5. DOI: 10.1248/bpb.26.1331. View

11.

Braca A, Sortino C, Politi M, Morelli I, Mendez J . Antioxidant activity of flavonoids from Licania licaniaeflora. J Ethnopharmacol. 2002; 79(3):379-81. DOI: 10.1016/s0378-8741(01)00413-5. View

12.

Mahakunakorn P, Tohda M, Murakami Y, Matsumoto K, Watanabe H . Antioxidant and free radical-scavenging activity of Choto-san and its related constituents. Biol Pharm Bull. 2004; 27(1):38-46. DOI: 10.1248/bpb.27.38. View

13.

Arivazhagan S, Balasenthil S, Nagini S . Garlic and neem leaf extracts enhance hepatic glutathione and glutathione dependent enzymes during N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced gastric carcinogenesis in rats. Phytother Res. 2000; 14(4):291-3. DOI: 10.1002/1099-1573(200006)14:4<291::aid-ptr570>3.0.co;2-y. View

14.

Miller M, Sadowska-Krowicka H, Chotinaruemol S, Kakkis J, Clark D . Amelioration of chronic ileitis by nitric oxide synthase inhibition. J Pharmacol Exp Ther. 1993; 264(1):11-6. View

15.

Bonaventura J, Schroeder W, Fang S . Human erythrocyte catalase: an improved method of isolation and a reevaluation of reported properties. Arch Biochem Biophys. 1972; 150(2):606-17. DOI: 10.1016/0003-9861(72)90080-x. View

16.

Beckman J, Chen J, Ischiropoulos H, Crow J . Oxidative chemistry of peroxynitrite. Methods Enzymol. 1994; 233:229-40. DOI: 10.1016/s0076-6879(94)33026-3. View

17.

Aruoma O, Halliwell B, Hoey B, Butler J . The antioxidant action of N-acetylcysteine: its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Radic Biol Med. 1989; 6(6):593-7. DOI: 10.1016/0891-5849(89)90066-x. View

18.

Halliwell B . Reactive oxygen species in living systems: source, biochemistry, and role in human disease. Am J Med. 1991; 91(3C):14S-22S. DOI: 10.1016/0002-9343(91)90279-7. View

19.

Bhattacharya A, Chatterjee A, Ghosal S, Bhattacharya S . Antioxidant activity of active tannoid principles of Emblica officinalis (amla). Indian J Exp Biol. 1999; 37(7):676-80. View

20.

Ischiropoulos H, Al-Mehdi A, Fisher A . Reactive species in ischemic rat lung injury: contribution of peroxynitrite. Am J Physiol. 1995; 269(2 Pt 1):L158-64. DOI: 10.1152/ajplung.1995.269.2.L158. View