Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2010 Apr 30

PMID 20428083

Citations 17

Authors

Jorge Lopez Garcia

Ahmad Asadinezhad

Jiri Pachernik

Marian Lehocky

Ita Junkar

Petr Humpolicek

Petr Saha

Pavel Valasek

Affiliations

Soon will be listed here.

Abstract

Argon plasma treatment was used to modify the surface of atelocollagen films using a plasmochemical reactor. To evaluate the effects of the treatment, the untreated and treated samples were characterized by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM) imaging, and X-ray Photoelectron Spectroscopy (XPS) techniques. Cell growth was carried out by culturing human immortalized keratinocyte (HaCaT) cells and proliferation was measured via MTT assay. It was observed that argon plasma treatment significantly enhanced the extent of cell proliferation, which was ascribed to the favourable role of plasma treatment in inducing surface oxygen-containing entities together with increasing surface roughness. This can be considered as a potentially promising approach for tissue regeneration purposes.

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References

Hauser J, Zietlow J, Koller M, Esenwein S, Halfmann H, Awakowicz P . Enhanced cell adhesion to silicone implant material through plasma surface modification. J Mater Sci Mater Med. 2009; 20(12):2541-8. DOI: 10.1007/s10856-009-3826-x. View

Boukamp P, Petrussevska R, Breitkreutz D, Hornung J, Markham A, Fusenig N . Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol. 1988; 106(3):761-71. PMC: 2115116. DOI: 10.1083/jcb.106.3.761. View

Maas-Szabowski N, Starker A, Fusenig N . Epidermal tissue regeneration and stromal interaction in HaCaT cells is initiated by TGF-alpha. J Cell Sci. 2003; 116(Pt 14):2937-48. DOI: 10.1242/jcs.00474. View

Mosmann T . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65(1-2):55-63. DOI: 10.1016/0022-1759(83)90303-4. View

Stanford C, Keller J, Solursh M . Bone cell expression on titanium surfaces is altered by sterilization treatments. J Dent Res. 1994; 73(5):1061-71. DOI: 10.1177/00220345940730050801. View

Prockop D, Kivirikko K . Collagens: molecular biology, diseases, and potentials for therapy. Annu Rev Biochem. 1995; 64:403-34. DOI: 10.1146/annurev.bi.64.070195.002155. View

Boelsma E, Verhoeven M, Ponec M . Reconstruction of a human skin equivalent using a spontaneously transformed keratinocyte cell line (HaCaT). J Invest Dermatol. 1999; 112(4):489-98. DOI: 10.1046/j.1523-1747.1999.00545.x. View

Zubavichus Y, Shaporenko A, Grunze M, Zharnikov M . Is X-ray absorption spectroscopy sensitive to the amino acid composition of functional proteins?. J Phys Chem B. 2008; 112(15):4478-80. DOI: 10.1021/jp801248n. View

DeSmet T, Morent R, De Geyter N, Leys C, Schacht E, Dubruel P . Nonthermal plasma technology as a versatile strategy for polymeric biomaterials surface modification: a review. Biomacromolecules. 2009; 10(9):2351-78. DOI: 10.1021/bm900186s. View

10.

Okuyama K, Xu X, Iguchi M, Noguchi K . Revision of collagen molecular structure. Biopolymers. 2005; 84(2):181-91. DOI: 10.1002/bip.20381. View

11.

Almazan-Almazan M, Paredes J, Perez-Mendoza M, Domingo-Garcia M, Lopez-Garzon F, Martinez-Alonso A . Surface characterisation of plasma-modified poly(ethylene terephthalate). J Colloid Interface Sci. 2005; 293(2):353-63. DOI: 10.1016/j.jcis.2005.06.073. View

12.

Kong J, Yu S . Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta Biochim Biophys Sin (Shanghai). 2007; 39(8):549-59. DOI: 10.1111/j.1745-7270.2007.00320.x. View

13.

Peschel G, Dahse H, Konrad A, Wieland G, Mueller P, Martin D . Growth of keratinocytes on porous films of poly(3-hydroxybutyrate) and poly(4-hydroxybutyrate) blended with hyaluronic acid and chitosan. J Biomed Mater Res A. 2007; 85(4):1072-81. DOI: 10.1002/jbm.a.31666. View

14.

Hu S, Jou C, Yang M . Protein adsorption, fibroblast activity and antibacterial properties of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) grafted with chitosan and chitooligosaccharide after immobilized with hyaluronic acid. Biomaterials. 2003; 24(16):2685-93. DOI: 10.1016/s0142-9612(03)00079-6. View

15.

Meineke V, Muller K, Ridi R, Cordes N, Kohn F, Mayerhofer A . Development and evaluation of a skin organ model for the analysis of radiation effects. Strahlenther Onkol. 2004; 180(2):102-8. DOI: 10.1007/s00066-004-1134-4. View

16.

OKUN L, Prado G, Applegate M, Ratcliffe A . Human articular chondrocyte adhesion and proliferation on synthetic biodegradable polymer films. Biomaterials. 1999; 20(23-24):2245-56. DOI: 10.1016/s0142-9612(99)00155-6. View

17.

Everaert E, van de Belt-Gritter B, van der Mei H, Busscher H, Verkerke G, Dijk F . In vitro and in vivo microbial adhesion and growth on argon plasma-treated silicone rubber voice prostheses. J Mater Sci Mater Med. 2004; 9(3):147-57. DOI: 10.1023/a:1008867619481. View

18.

Paleos C, Tsiourvas D, Sideratou Z . Hydrogen bonding interactions of liposomes simulating cell-cell recognition. A review. Orig Life Evol Biosph. 2004; 34(1-2):195-213. DOI: 10.1023/b:orig.0000009840.53758.c2. View

19.

Lehmann B . HaCaT cell line as a model system for vitamin D3 metabolism in human skin. J Invest Dermatol. 1997; 108(1):78-82. DOI: 10.1111/1523-1747.ep12285640. View

20.

Schumacher M, Mizuno K, Bachinger H . The crystal structure of the collagen-like polypeptide (glycyl-4(R)-hydroxyprolyl-4(R)-hydroxyprolyl)9 at 1.55 A resolution shows up-puckering of the proline ring in the Xaa position. J Biol Chem. 2005; 280(21):20397-403. DOI: 10.1074/jbc.M501453200. View