Polyphenolic Extracts of Edible Flowers Incorporated Onto Atelocollagen Matrices and Their Effect on Cell Viability

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2013 Nov 2

PMID 24177700

Citations 5

Authors

Jorge Lopez-Garcia

Zdenka Kucekova

Petr Humpolicek

Jiri Mlcek

Petr Saha

Affiliations

Soon will be listed here.

Abstract

The phenolic extract of chives flowers (Allium schoenoprasum, Liliaceae), introduced Sage (Salvia pratensis, Lamiaceae), European elderberry (Sambucus nigra, Caprifoliaceae) and common dandelion (Taraxacum officinale, Asteraceae) were characterised by High Performance Liquid Chromatography and incorporated in different concentrations onto atelocollagen thin films. In order to assess the biological impact of these phenolic compounds on cell viability, human immortalised non-tumorigenic keratinocyte cell line was seeded on the thin films and cell proliferation was determined by using an MTT assay. In addition, their antimicrobial activity was estimated by using an agar diffusion test. Data indicated the concomitance between cell viability and concentration of polyphenols. These findings suggest that these phenolic-endowed atelocollagen films might be suitable for tissue engineering applications, on account of the combined activity of polyphenols and collagen.

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