Protein Adsorption on Various Plasma-treated Polyethylene Terephthalate Substrates

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2013 Oct 25

PMID 24152668

Citations 14

Authors

Nina Recek

Morana Jaganjac

Metod Kolar

Lidija Milkovic

Miran Mozetic

Karin Stana-Kleinschek

Alenka Vesel

Affiliations

Soon will be listed here.

Abstract

Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF(4) plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy (SEM). The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates.

Citing Articles

Progress and Outlook on Electrochemical Sensing of Lung Cancer Biomarkers.

Zheng R, Wu A, Li J, Tang Z, Zhang J, Zhang M Molecules. 2024; 29(13).

PMID: 38999110 PMC: 11243195. DOI: 10.3390/molecules29133156.

Performance and biocompatibility of OSTEMER 322 in cell-based microfluidic applications.

Aubrecht P, Smejkal J, Panuska P, Spanbauerova K, Neubertova V, Kaule P RSC Adv. 2024; 14(6):3617-3635.

PMID: 38268545 PMC: 10804231. DOI: 10.1039/d3ra05789e.

Integration of Extracellular Matrices into Organ-on-Chip Systems.

Kutluk H, Bastounis E, Constantinou I Adv Healthc Mater. 2023; 12(20):e2203256.

PMID: 37018430 PMC: 11468608. DOI: 10.1002/adhm.202203256.

A Novel Hydrophilic, Antibacterial Chitosan-Based Coating Prepared by Ultrasonic Atomization Assisted LbL Assembly Technique.

Wang X, Zhou Y, Johnson M, Milne C, A S, Li Y J Funct Biomater. 2023; 14(1).

PMID: 36662091 PMC: 9863527. DOI: 10.3390/jfb14010043.

Surface Properties of the Polyethylene Terephthalate (PET) Substrate Modified with the Phospholipid-Polypeptide-Antioxidant Films: Design of Functional Biocoatings.

Szafran K, Jurak M, Mroczka R, Wiacek A Pharmaceutics. 2022; 14(12).

PMID: 36559307 PMC: 9780983. DOI: 10.3390/pharmaceutics14122815.

References

Flemming R, Murphy C, Abrams G, Goodman S, Nealey P . Effects of synthetic micro- and nano-structured surfaces on cell behavior. Biomaterials. 1999; 20(6):573-88. DOI: 10.1016/s0142-9612(98)00209-9. View

Popelka A, Novak I, Lehocky M, Junkar I, Mozetic M, Kleinova A . A new route for chitosan immobilization onto polyethylene surface. Carbohydr Polym. 2012; 90(4):1501-8. DOI: 10.1016/j.carbpol.2012.07.021. View

Blackstone B, Willard J, Lee C, Nelson M, Hart R, Lannutti J . Plasma surface modification of electrospun fibers for adhesion-based cancer cell sorting. Integr Biol (Camb). 2012; 4(9):1112-21. DOI: 10.1039/c2ib20025b. View

Martinez E, Engel E, Planell J, Samitier J . Effects of artificial micro- and nano-structured surfaces on cell behaviour. Ann Anat. 2008; 191(1):126-35. DOI: 10.1016/j.aanat.2008.05.006. View

DeSmet T, Morent R, De Geyter N, Leys C, Schacht E, Dubruel P . Nonthermal plasma technology as a versatile strategy for polymeric biomaterials surface modification: a review. Biomacromolecules. 2009; 10(9):2351-78. DOI: 10.1021/bm900186s. View

Lord M, Modin C, Foss M, Duch M, Simmons A, Pedersen F . Monitoring cell adhesion on tantalum and oxidised polystyrene using a quartz crystal microbalance with dissipation. Biomaterials. 2006; 27(26):4529-37. DOI: 10.1016/j.biomaterials.2006.04.006. View

Doliska A, Ribitsch V, Kleinschek K, Strnad S . Viscoelastic properties of fibrinogen adsorbed onto poly(ethylene terephthalate) surfaces by QCM-D. Carbohydr Polym. 2013; 93(1):246-55. DOI: 10.1016/j.carbpol.2012.02.075. View

Kim Y, Kang I, Huh M, Yoon S . Surface characterization and in vitro blood compatibility of poly(ethylene terephthalate) immobilized with insulin and/or heparin using plasma glow discharge. Biomaterials. 2000; 21(2):121-30. DOI: 10.1016/s0142-9612(99)00137-4. View

Garcia J, Asadinezhad A, Pachernik J, Lehocky M, Junkar I, Humpolicek P . Cell proliferation of HaCaT keratinocytes on collagen films modified by argon plasma treatment. Molecules. 2010; 15(4):2845-56. PMC: 6257401. DOI: 10.3390/molecules15042845. View

10.

Anand G, Sharma S, Dutta A, Kumar S, Belfort G . Conformational transitions of adsorbed proteins on surfaces of varying polarity. Langmuir. 2010; 26(13):10803-11. DOI: 10.1021/la1006132. View

11.

Popelka A, Novak I, Lehocky M, Chodak I, Sedliacik J, Gajtanska M . Anti-bacterial treatment of polyethylene by cold plasma for medical purposes. Molecules. 2012; 17(1):762-85. PMC: 6268539. DOI: 10.3390/molecules17010762. View

12.

DSa R, Burke G, Meenan B . Protein adhesion and cell response on atmospheric pressure dielectric barrier discharge-modified polymer surfaces. Acta Biomater. 2010; 6(7):2609-20. DOI: 10.1016/j.actbio.2010.01.015. View

13.

Fong E, Lamhamedi-Cherradi S, Burdett E, Ramamoorthy V, Lazar A, Kasper F . Modeling Ewing sarcoma tumors in vitro with 3D scaffolds. Proc Natl Acad Sci U S A. 2013; 110(16):6500-5. PMC: 3631678. DOI: 10.1073/pnas.1221403110. View

14.

Dowling D, Miller I, Ardhaoui M, Gallagher W . Effect of surface wettability and topography on the adhesion of osteosarcoma cells on plasma-modified polystyrene. J Biomater Appl. 2010; 26(3):327-47. DOI: 10.1177/0885328210372148. View

15.

Rabe M, Verdes D, Seeger S . Understanding protein adsorption phenomena at solid surfaces. Adv Colloid Interface Sci. 2011; 162(1-2):87-106. DOI: 10.1016/j.cis.2010.12.007. View

16.

Asadinezhad A, Novak I, Lehocky M, Sedlarik V, Vesel A, Junkar I . An in vitro bacterial adhesion assessment of surface-modified medical-grade PVC. Colloids Surf B Biointerfaces. 2010; 77(2):246-56. DOI: 10.1016/j.colsurfb.2010.02.006. View

17.

Dixon M . Quartz crystal microbalance with dissipation monitoring: enabling real-time characterization of biological materials and their interactions. J Biomol Tech. 2009; 19(3):151-8. PMC: 2563918. View

18.

Xu T, Fu R, Yan L . A new insight into the adsorption of bovine serum albumin onto porous polyethylene membrane by zeta potential measurements, FTIR analyses, and AFM observations. J Colloid Interface Sci. 2005; 262(2):342-50. DOI: 10.1016/S0021-9797(03)00208-X. View

19.

Jaganjac M, Matijevic T, Cindric M, Cipak A, Mrakovcic L, Gubisch W . Induction of CMV-1 promoter by 4-hydroxy-2-nonenal in human embryonic kidney cells. Acta Biochim Pol. 2010; 57(2):179-83. View

20.

Ramires P, Mirenghi L, Romano A, Palumbo F, Nicolardi G . Plasma-treated PET surfaces improve the biocompatibility of human endothelial cells. J Biomed Mater Res. 2000; 51(3):535-9. DOI: 10.1002/1097-4636(20000905)51:3<535::aid-jbm31>3.0.co;2-p. View