Nucleoredoxin Negatively Regulates Toll-like Receptor 4 Signaling Via Recruitment of Flightless-I to Myeloid Differentiation Primary Response Gene (88)

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2010 Apr 20

PMID 20400501

Citations 21

Authors

Tatsuya Hayashi

Yosuke Funato

Takeshi Terabayashi

Akifumi Morinaka

Reiko Sakamoto

Hirotake Ichise

Hiroyuki Fukuda

Nobuaki Yoshida

Hiroaki Miki

Affiliations

Soon will be listed here.

Abstract

We previously characterized nucleoredoxin (NRX) as a negative regulator of the Wnt signaling pathway through Dishevelled (Dvl). We perform a comprehensive search for other NRX-interacting proteins and identify Flightless-I (Fli-I) as a novel NRX-binding partner. Fli-I binds to NRX and other related proteins, such as Rod-derived cone viability factor (RdCVF), whereas Dvl binds only to NRX. Endogenous NRX and Fli-I in vivo interactions are confirmed. Both NRX and RdCVF link Fli-I with myeloid differentiation primary response gene (88) (MyD88), an important adaptor protein for innate immune response. NRX and RdCVF also potentiate the negative effect of Fli-I upon lipopolysaccharide-induced activation of NF-kappaB through the Toll-like receptor 4/MyD88 pathway. Embryonic fibroblasts derived from NRX gene-targeted mice show aberrant NF-kappaB activation upon lipopolysaccharide stimulation. These results suggest that the NRX subfamily of proteins forms a link between MyD88 and Fli-I to mediate negative regulation of the Toll-like receptor 4/MyD88 pathway.

Citing Articles

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