MiRTRAP, a Computational Method for the Systematic Identification of MiRNAs from High Throughput Sequencing Data

Overview

Journal Genome Biol

Specialties Biology
Genetics

Date 2010 Apr 8

PMID 20370911

Citations 49

Authors

David Hendrix

Michael Levine

Weiyang Shi

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRs) have been broadly implicated in animal development and disease. We developed a novel computational strategy for the systematic, whole-genome identification of miRs from high throughput sequencing information. This method, miRTRAP, incorporates the mechanisms of miR biogenesis and includes additional criteria regarding the prevalence and quality of small RNAs arising from the antisense strand and neighboring loci. This program was applied to the simple chordate Ciona intestinalis and identified nearly 400 putative miR loci.

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