Convertase Inhibitory Properties of Staphylococcal Extracellular Complement-binding Protein

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2010 Mar 23

PMID 20304920

Citations 22

Authors

Ilse Jongerius

Brandon L Garcia

Brian V Geisbrecht

Jos A G van Strijp

Suzan H M Rooijakkers

Affiliations

Soon will be listed here.

Abstract

The human pathogen Staphylococcus aureus secretes several complement evasion molecules to combat the human immune response. Extracellular complement-binding protein (Ecb) binds to the C3d domain of C3 and thereby blocks C3 convertases of the alternative pathway and C5 convertases via all complement pathways. Inhibition of C5 convertases results in complete inhibition of C5a generation and subsequent neutrophil migration. Here, we show that binding of Ecb to the C3d domain of C3b is crucial for inhibition of C5 convertases. Ecb does not interfere with substrate binding to convertases but prevents formation of an active convertase enzyme.

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