Photoreceptor Cell Degeneration in Abcr (-/-) Mice

Overview

Journal Adv Exp Med Biol

Specialties Biology
General Medicine

Date 2010 Mar 19

PMID 20238056

Citations 53

Authors

Li Wu

Taka Nagasaki

Janet R Sparrow

Affiliations

Soon will be listed here.

Abstract

Mice harboring a null mutation in Abca4/Abcr serve as a model of autosomal recessive Stargardt disease. Consistent with the human retinal disorder, deficiency in Abcr is associated with substantial accumulations of lipofuscin pigments in retinal pigment epithelial (RPE) cells. To observe for photoreceptor cell degeneration in these mutant mice, outer nuclear layer (ONL) thickness was measured at 200 mum intervals superior and inferior to the optic nerve head. ONL width in Abcr (-/-) mouse was reduced at 8-9 month and 11 and 13 months relative to Abcr ( +/+ ) mice; thinning was more pronounced centrally and in superior retina. The numbers of photoreceptor nuclei spanning the width of the outer nuclear layer were also reduced. No evidence of age-related ONL thinning was observed in Abcr (+/+) mice at these ages. We conclude that albino Abcr (-/-) mice exhibit progressive photoreceptor cell loss that is detectable at 8 months of age and that has worsened by 11 and 13 months of age. The measurement of ONL thickness is an established approach to assessing photoreceptor cell integrity and can be used in preclinical studies using Abcr (-/-) mice.

Citing Articles

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