Ring-shaped Rad51 Paralog Protein Complexes Bind Holliday Junctions and Replication Forks As Visualized by Electron Microscopy

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2010 Mar 9

PMID 20207730

Citations 16

Authors

Sarah A Compton

Sezgin Ozgur

Jack D Griffith

Affiliations

Soon will be listed here.

Abstract

In mammals, there are five Rad51 paralogs that form two distinct complexes in vivo. One complex is composed of Rad51B-Rad51C-Rad51D-Xrcc2 (BCDX2) and the other Rad51C-Xrcc3 (CX3). We co-expressed and purified human BCDX2 and CX3 protein complexes from insect cells and investigated their binding preferences and structure using transmission electron microscopy (TEM). We visualized the binding of BCDX2 and CX3 to DNA templates containing replication forks and Holliday junctions, intermediates observed during DNA replication and recombination, respectively. We show that both complexes bind with exceptionally high specificity to the DNA junctions with little binding observed elsewhere on the DNAs. Further analysis of the structure of free or DNA-bound BCDX2 and CX3 complexes revealed a multimeric ring structure whose subunits are arranged into a flat disc around a central channel. This work provides the first EM visualization of BCDX2 and CX3 binding to Holliday junctions and forked DNAs and suggests the complexes form ring-shaped structures.

Citing Articles

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Novel insights into RAD51 activity and regulation during homologous recombination and DNA replication.

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Mammalian RAD51 paralogs protect nascent DNA at stalled forks and mediate replication restart.

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Roles of Rad51 paralogs for promoting homologous recombination in Leishmania infantum.

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Overexpression of Rad51C splice variants in colorectal tumors.

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