Tracing Conformational Transition of Abnormal Prion Proteins During Interspecies Transmission by Using Novel Antibodies

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2010 Feb 24

PMID 20177064

Citations 13

Authors

Yuko Ushiki-Kaku

Ryo Endo

Yoshifumi Iwamaru

Yoshihisa Shimizu

Morikazu Imamura

Kentaro Masujin

Takuji Yamamoto

Shunji Hattori

Shigeyoshi Itohara

Shinkichi Irie

Takashi Yokoyama

Affiliations

Soon will be listed here.

Abstract

Conformational differences in abnormal prion proteins (PrP(Sc)) have been postulated to produce different prion phenotypes. During the interspecies transmission of prions, the conformation of PrP(Sc) may change with passage; however, little is known about the mechanism of PrP(Sc) transition. In this study, novel PrP(Sc)-specific monoclonal antibodies (mAbs) were developed that could detect the PrP(Sc) of mouse but not that of sheep. By using these mAbs, we attempted to examine PrP(Sc) accumulated in mice inoculated with sheep scrapie serially up to five passages. The presence of PrP(Sc) in the mice was confirmed at all passages; however, mAb-bound PrP(Sc) conformer was detected only from the third passage onward. The generated mAb enabled tracing of a particular conformer during adaptation in sheep-to-mice transmission of prion, suggesting that the conformational transition of PrP(Sc) was caused by propagation of this conformer. Such mAbs capable of discriminating conformational differences may allow us to address questions concerning PrP(Sc) conformation and strain diversity.

Citing Articles

Enhancement of binding avidity by bivalent binding enables PrPSc-specific detection by anti-PrP monoclonal antibody 132.

Suzuki A, Yamasaki T, Hasebe R, Horiuchi M PLoS One. 2019; 14(6):e0217944.

PMID: 31170247 PMC: 6553756. DOI: 10.1371/journal.pone.0217944.

Self-propagating, protease-resistant, recombinant prion protein conformers with or without in vivo pathogenicity.

Wang F, Wang X, Orru C, Groveman B, Surewicz K, Abskharon R PLoS Pathog. 2017; 13(7):e1006491.

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Selective propagation of mouse-passaged scrapie prions with long incubation period from a mixed prion population using GT1-7 cells.

Miyazawa K, Masujin K, Okada H, Ushiki-Kaku Y, Matsuura Y, Yokoyama T PLoS One. 2017; 12(6):e0179317.

PMID: 28636656 PMC: 5479544. DOI: 10.1371/journal.pone.0179317.

Comparison of abnormal isoform of prion protein in prion-infected cell lines and primary-cultured neurons by PrPSc-specific immunostaining.

Tanaka M, Fujiwara A, Suzuki A, Yamasaki T, Hasebe R, Masujin K J Gen Virol. 2016; 97(8):2030-2042.

PMID: 27267758 PMC: 5770843. DOI: 10.1099/jgv.0.000514.

PrPSc-Specific Antibody Reveals C-Terminal Conformational Differences between Prion Strains.

Saijo E, Hughson A, Raymond G, Suzuki A, Horiuchi M, Caughey B J Virol. 2016; 90(10):4905-4913.

PMID: 26937029 PMC: 4859706. DOI: 10.1128/JVI.00088-16.

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