Microglial Cell Line Established from Prion Protein-overexpressing Mice is Susceptible to Various Murine Prion Strains

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2006 Nov 24

PMID 17121794

Citations 27

Authors

Yoshifumi Iwamaru

Takato Takenouchi

Kazumasa Ogihara

Megumi Hoshino

Masuhiro Takata

Morikazu Imamura

Yuichi Tagawa

Hiroko Hayashi-Kato

Yuko Ushiki-Kaku

Yoshihisa Shimizu

Hiroyuki Okada

Morikazu Shinagawa

Hiroshi Kitani

Takashi Yokoyama

Affiliations

Soon will be listed here.

Abstract

Several lines of evidence suggest that microglia have important roles in the pathogenesis of prion diseases. Here, we establish a novel microglial cell line (MG20) from neonatal tga20 mice that overexpress murine prion protein. After exposure to Chandler scrapie, we observed the replication and accumulation of disease-associated forms of the prion protein in MG20 cells up to the 15th passage. Furthermore, MG20 cells were susceptible to ME7, Obihiro scrapie, and bovine spongiform encephalopathy agents. Thus, MG20 cell lines persistently infected with various murine prion strains provide a useful model for the study of the pathogenesis of prion diseases.

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