Molecular Genetic Analysis of the Orsellinic Acid/F9775 Gene Cluster of Aspergillus Nidulans

Overview

Journal Mol Biosyst

Publisher Royal Society of Chemistry

Specialties Biochemistry
Molecular Biology

Date 2010 Feb 23

PMID 20174687

Citations 61

Authors

James F Sanchez

Yi-Ming Chiang

Edyta Szewczyk

Ashley D Davidson

Manmeet Ahuja

C Elizabeth Oakley

Jin Woo Bok

Nancy Keller

Berl R Oakley

Clay C C Wang

Affiliations

Soon will be listed here.

Abstract

F-9775A and F-9775B are cathepsin K inhibitors that arise from a chromatin remodelling deletant strain of Aspergillus nidulans. A polyketide synthase gene has been determined to be responsible for their formation and for the simpler, archetypical polyketide orsellinic acid. We have discovered simple culture conditions that result in the production of the three compounds, and this facilitates analysis of the genes responsible for their synthesis. We have now analysed the F9775/orsellinic acid gene cluster using a set of targeted deletions. We find that the polyketide synthase alone is required for orsellinic acid biosynthesis and only two additional genes in the cluster are required for F9775 A and B synthesis. Our deletions also yielded the bioactive metabolites gerfelin and diorcinol.

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