Activity-based Proteomics of Enzyme Superfamilies: Serine Hydrolases As a Case Study

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2010 Feb 12

PMID 20147750

Citations 143

Authors

Gabriel M Simon

Benjamin F Cravatt

Affiliations

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Abstract

Genome sequencing projects have uncovered thousands of uncharacterized enzymes in eukaryotic and prokaryotic organisms. Deciphering the physiological functions of enzymes requires tools to profile and perturb their activities in native biological systems. Activity-based protein profiling has emerged as a powerful chemoproteomic strategy to achieve these objectives through the use of chemical probes that target large swaths of enzymes that share active-site features. Here, we review activity-based protein profiling and its implementation to annotate the enzymatic proteome, with particular attention given to probes that target serine hydrolases, a diverse superfamily of enzymes replete with many uncharacterized members.

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