A Nonviral Minicircle Vector for Deriving Human IPS Cells

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2010 Feb 9

PMID 20139967

Citations 326

Authors

Fangjun Jia

Kitchener D Wilson

Ning Sun

Deepak M Gupta

Mei Huang

Zongjin Li

Nicholas J Panetta

Zhi Ying Chen

Robert C Robbins

Mark A Kay

Michael T Longaker

Joseph C Wu

Affiliations

Soon will be listed here.

Abstract

Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.

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