Taenia Saginata Metacestode Antigenic Fractions Without Affinity to Concanavalin A Are an Important Source of Specific Antigens for the Diagnosis of Human Neurocysticercosis

Overview

Journal Clin Vaccine Immunol

Publisher American Society for Microbiology

Specialties Allergy & Immunology
Pathology

Date 2010 Feb 5

PMID 20130125

Citations 3

Authors

Heliana B Oliveira

Gleyce A Machado

Jose R Mineo

Julia M Costa-Cruz

Affiliations

Soon will be listed here.

Abstract

Taenia saginata metacestode antigens have been constituted a useful alternative antigen for neurocysticercosis (NC) serodiagnosis, particularly due to an increasing difficulty to obtain Taenia solium homologous antigen. Cross-reactivity with Echinococcus granulosus infection occurs in homologous and heterologous antigens and could be avoided by using different purified methods. The present study evaluated antigen fractions obtained from saline extracts of T. saginata metacestodes purified by affinity chromatography with jacalin or concanavalin A (ConA) lectins to detect IgG antibodies by enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis to diagnose human NC. Serum samples were collected from 142 individuals: 40 of them were diagnosed with NC, 62 presented Taenia sp. and other parasites, and 40 were apparently healthy individuals. The jacalin- and ConA-unbound fractions demonstrated sensitivity and specificity higher than those of bound fractions. Among unbound fractions, ConA demonstrated statistically higher sensitivity and specificity by ELISA (90% and 93.1%, respectively). By immunoblot assay, the 64- to 68-kDa component from the ConA-unbound fraction showed 100% sensitivity and specificity, making this component suitable for use as a specific antigen for diagnosis of NC. To our knowledge, this is the first report showing the relevance of using the unbound ConA fraction of T. saginata metacestodes to diagnose NC. In conclusion, the results obtained herein clearly demonstrate that antigenic fractions without affinity to ConA, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC when tested by immunoblot assay.

Citing Articles

Diethylaminoethyl (DEAE) binding fraction from Taenia solium metacestode improves the neurocysticercosis serodiagnosis.

Ribeiro V, Nunes D, Gonzaga H, da Cunha-Junior J, Costa-Cruz J Parasitol Res. 2014; 113(7):2569-75.

PMID: 24777340 DOI: 10.1007/s00436-014-3908-7.

An immunoblot for detection of Taenia saginata cysticercosis.

Abuseir S, Nagel-Kohl U, Wolken S, Strube C Parasitol Res. 2013; 112(5):2069-73.

PMID: 23483261 DOI: 10.1007/s00436-013-3368-5.

Wormy surprise!.

Jesudoss A, Kaya M, Lisa S, Rohatgi A BMJ Case Rep. 2012; 2012.

PMID: 22729345 PMC: 3387467. DOI: 10.1136/bcr.12.2011.5416.

References

Montenegro T, Gilman R, Castillo R, Tsang V, Brandt J, Guevara A . The diagnostic importance of species specific and cross-reactive components of Taenia solium, Echinococcus granulosus, and Hymenolepis nana. Rev Inst Med Trop Sao Paulo. 1994; 36(4):327-34. DOI: 10.1590/s0036-46651994000400005. View

Oliveira H, Machado G, Goncalves-Pires M, Moura L, Costa-Cruz J . Saline extract of Taenia saginata metacestodes as an alternative antigen for the immunodiagnosis of neurocysticercosis in human cerebrospinal fluid. Parasitol Res. 2009; 105(1):169-74. DOI: 10.1007/s00436-009-1379-z. View

Morakote N, Nawacharoen W, Sukonthasun K, Thammasonthi W, Khamboonruang C . Comparison of cysticercus extract, cyst fluid and Taenia saginata extract for use in ELISA for serodiagnosis of neurocysticercosis. Southeast Asian J Trop Med Public Health. 1992; 23(1):77-81. View

Jeyaprakash A, Jayashree G, Mahanta S, Swaminathan C, Sekar K, Surolia A . Structural basis for the energetics of jacalin-sugar interactions: promiscuity versus specificity. J Mol Biol. 2005; 347(1):181-8. DOI: 10.1016/j.jmb.2005.01.015. View

SUMNER J, HOWELL S . Identification of Hemagglutinin of Jack Bean with Concanavalin A. J Bacteriol. 1936; 32(2):227-37. PMC: 543784. DOI: 10.1128/jb.32.2.227-237.1936. View

Sharon N, Lis H . Lectins: cell-agglutinating and sugar-specific proteins. Science. 1972; 177(4053):949-59. DOI: 10.1126/science.177.4053.949. View

Ferrer E, Gonzalez L, Martinez-Escribano J, Gonzalez-Barderas M, Cortez M, Davila I . Evaluation of recombinant HP6-Tsag, an 18 kDa Taenia saginata oncospheral adhesion protein, for the diagnosis of cysticercosis. Parasitol Res. 2007; 101(3):517-25. DOI: 10.1007/s00436-007-0507-x. View

Del Brutto O, Rajshekhar V, White Jr A, Tsang V, Nash T, Takayanagui O . Proposed diagnostic criteria for neurocysticercosis. Neurology. 2001; 57(2):177-83. PMC: 2912527. DOI: 10.1212/wnl.57.2.177. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

10.

Kong Y, Kang S, Cho S, Min D . Cross-reacting and specific antigenic components in cystic fluid from metacestodes of Echinococcus granulosus and Taenia solium. Kisaengchunghak Chapchi. 1989; 27(2):131-9. DOI: 10.3347/kjp.1989.27.2.131. View

11.

Friedman R . Comparison of four different silver-staining techniques for salivary protein detection in alkaline polyacrylamide gels. Anal Biochem. 1982; 126(2):346-9. DOI: 10.1016/0003-2697(82)90525-5. View

12.

Del Brutto O, Wadia N, Dumas M, Cruz M, Tsang V, Schantz P . Proposal of diagnostic criteria for human cysticercosis and neurocysticercosis. J Neurol Sci. 1996; 142(1-2):1-6. DOI: 10.1016/0022-510x(96)00130-x. View

13.

Zambelli J . The hyperinsulinemia produced by concanavalin A in rats is opioid-dependent and hormonally regulated. Braz J Med Biol Res. 1998; 31(5):697-703. DOI: 10.1590/s0100-879x1998000500015. View

14.

Hancock K, Pattabhi S, Greene R, Yushak M, Williams F, Khan A . Characterization and cloning of GP50, a Taenia solium antigen diagnostic for cysticercosis. Mol Biochem Parasitol. 2003; 133(1):115-24. DOI: 10.1016/j.molbiopara.2003.10.001. View

15.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

16.

Tsang V, Brand J, Boyer A . An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis. 1989; 159(1):50-9. DOI: 10.1093/infdis/159.1.50. View

17.

Barbieri M, Fernandez V, Gonzalez G, Luaces V, Nieto A . Diagnostic evaluation of a synthetic peptide derived from a novel antigen B subunit as related to other available peptides and native antigens used for serology of cystic hydatidosis. Parasite Immunol. 1998; 20(2):51-61. DOI: 10.1046/j.1365-3024.1998.00117.x. View

18.

Youden W . Index for rating diagnostic tests. Cancer. 1950; 3(1):32-5. DOI: 10.1002/1097-0142(1950)3:1<32::aid-cncr2820030106>3.0.co;2-3. View

19.

Husain N, Shukla N, Kumar R, Husain M, Chaturvedi A, Agarwal G . ELISA in the evaluation of therapeutic response to albendazole in neurocysticercosis. J Infect. 2007; 56(1):65-73. DOI: 10.1016/j.jinf.2007.09.014. View

20.

Oliveira H, Rodrigues R, Barcelos I, Silva L, Costa-Cruz J . Anti-Taenia solium metacestode IgG antibodies in serum samples from inhabitants of a central-western region of Brazil. Rev Inst Med Trop Sao Paulo. 2006; 48(1):49-52. DOI: 10.1590/s0036-46652006000100011. View