Saline Extract of Taenia Saginata Metacestodes As an Alternative Antigen for the Immunodiagnosis of Neurocysticercosis in Human Cerebrospinal Fluid

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2009 Feb 28

PMID 19247689

Citations 3

Authors

Heliana B Oliveira

Gleyce A Machado

Maria Do Rosario F Goncalves-Pires

Leandro P Moura

Julia M Costa-Cruz

Affiliations

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Abstract

The aim of the present research was to test the application of Taenia saginata metacestodes as an alternative antigen for use in enzyme-linked immunosorbent assay (ELISA) and Western Blotting (WB) tests compared with the use of metacestodes antigen of Taenia solium in cerebrospinal fluid (CSF) samples. The samples were obtained from 35 patients with definitive neurocysticercosis (NCC)-group 1-and 44 patients with other neurological disorders (control)-group 2. The sensitivity and specificity of ELISA, using antigen obtained from T. solium, applied to the patients of group 1 yielded results of 100%. When the tests were conducted using T. saginata metacestodes, results were 100% and 93.2%, respectively. The 47-52-, 64-68-, and 70-kDa antigens showed high frequencies in CSF samples from patients with NCC when WB was conducted with both antigens. The results indicate that T. saginata metacestodes can be used as an alternative antigen for the diagnosis of human NCC in CSF samples.

Citing Articles

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Wu W, Jia F, Wang W, Huang Y, Huang Y Parasitol Res. 2013; 112(8):2879-90.

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Immunodiagnosis of neurocysticercosis: ways to focus on the challenge.

Esquivel-Velazquez M, Ostoa-Saloma P, Morales-Montor J, Hernandez-Bello R, Larralde C J Biomed Biotechnol. 2011; 2011:516042.

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Taenia saginata metacestode antigenic fractions without affinity to concanavalin A are an important source of specific antigens for the diagnosis of human neurocysticercosis.

Oliveira H, Machado G, Mineo J, Costa-Cruz J Clin Vaccine Immunol. 2010; 17(4):638-44.

PMID: 20130125 PMC: 2849342. DOI: 10.1128/CVI.00516-09.

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