Diethylaminoethyl (DEAE) Binding Fraction from Taenia Solium Metacestode Improves the Neurocysticercosis Serodiagnosis

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2014 Apr 30

PMID 24777340

Citations 1

Authors

Vanessa da S Ribeiro

Daniela da S Nunes

Henrique T Gonzaga

Jair P da Cunha-Junior

Julia M Costa-Cruz

Affiliations

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Abstract

Neurocysticercosis (NC) is one of the most important diseases caused by parasites affecting the central nervous system. We fractionated by ion-exchange chromatography using diethylaminoethyl (DEAE)-sepharose resin the total saline extract (S) from Taenia solium metacestodes and evaluated obtained fractions (DEAE S1 and DEAE S2) by enzyme-linked immunosorbent assay (ELISA, n = 123) and immunoblotting (IB, n = 22) to detect human NC in serum. Diagnostic parameters were established by ROC and TG ROC curves for ELISA tests. IB was qualitatively analyzed. S and DEAE S1 presented sensitivity of 87. 5% and DEAE S2 90%. The best specificity was observed for DEAE S2 (90.4%). In IB, using DEAE S2 samples from NC patients presented bands of 20-25, 43-45, 55-50, 60-66, 82, 89, and 140 kDa. The great diagnostic parameters reached by DEAE S2 suggest the potential applicability of this fraction in NC immunodiagnosis.

Citing Articles

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