Target-enrichment Strategies for Next-generation Sequencing

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2010 Jan 30

PMID 20111037

Citations 478

Authors

Lira Mamanova

Alison J Coffey

Carol E Scott

Iwanka Kozarewa

Emily H Turner

Akash Kumar

Eleanor Howard

Jay Shendure

Daniel J Turner

Affiliations

Soon will be listed here.

Abstract

We have not yet reached a point at which routine sequencing of large numbers of whole eukaryotic genomes is feasible, and so it is often necessary to select genomic regions of interest and to enrich these regions before sequencing. There are several enrichment approaches, each with unique advantages and disadvantages. Here we describe our experiences with the leading target-enrichment technologies, the optimizations that we have performed and typical results that can be obtained using each. We also provide detailed protocols for each technology so that end users can find the best compromise between sensitivity, specificity and uniformity for their particular project.

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