Alpha 2.0
Login Register
» Articles » PMID: 20105295

Detection of Dengue Virus in Saliva and Urine by Real Time RT-PCR

Overview
Journal Virol J
Publisher Biomed Central
Specialty Microbiology
Date 2010 Jan 29
PMID 20105295
Citations 40
Authors
Telma R Poloni
Anibal S Oliveira
Helda L Alfonso
Larissa R Galvao
Alberto A Amarilla
Dimair F Poloni
Luiz T Figueiredo
Victor H Aquino
Affiliations
Soon will be listed here.
Abstract

Early diagnosis of dengue virus (DENV) infection is important for patient management and control of dengue outbreaks. The objective of this study was to analyze the usefulness of urine and saliva samples for early diagnosis of DENV infection by real time RT-PCR. Two febrile patients, who have been attended at the General Hospital of the School of Medicine of Ribeirao Preto, Sao Paulo University were included in the study. Serum, urine and saliva samples collected from both patients were subjected to real time RT-PCR for DENV detection and quantification. Dengue RNA was detected in serum, urine and saliva samples of both patients. Patient 1 was infected with DENV-2 and patient 2 with DENV-3. Data presented in this study suggest that urine and saliva could be used as alternative samples for early diagnosis of dengue virus infection when blood samples are difficult to obtain, e.g., in newborns and patients with hemorrhagic syndromes.

Citing Articles

Can Wastewater Surveillance Enhance Genomic Tracking of Climate-Driven Pathogens?.

Van Poelvoorde L, Karlsson E, Dupont-Rouzeyrol M, Roosens N Microorganisms. 2025; 13(2).

PMID: 40005661 PMC: 11858121. DOI: 10.3390/microorganisms13020294.

A Perspective on Wastewater and Environmental Surveillance as a Public Health Tool for Low- and Middle-Income Countries.

Khan M, Wurzbacher C, Uchaikina A, Pleshkov B, Mirshina O, Drewes J Microorganisms. 2025; 13(2).

PMID: 40005606 PMC: 11857276. DOI: 10.3390/microorganisms13020238.

Direct detection and identification of viruses in saliva using a SpecID ionization modified mass spectrometer.

Alusta P, Paredes A, Azevedo M, Mullis L, Buzatu D PLoS One. 2025; 20(2):e0316368.

PMID: 39919111 PMC: 11805448. DOI: 10.1371/journal.pone.0316368.

Neuro-Ophthalmic Dengue Infection: A Case Report with a Multiple Body Site Sampling Strategy and Review of Laboratory Data.

Butel-Simoes G, Bajaj N, Asad S, Moselen J, Orlando N, Steinig E Viruses. 2024; 16(7.

PMID: 39066161 PMC: 11281680. DOI: 10.3390/v16070998.

The Role of NS1 Protein in the Diagnosis of Flavivirus Infections.

Fisher R, Lustig Y, Sklan E, Schwartz E Viruses. 2023; 15(2).

PMID: 36851784 PMC: 9963814. DOI: 10.3390/v15020572.

References
1.
Halstead S . Dengue. Lancet. 2007; 370(9599):1644-52. DOI: 10.1016/S0140-6736(07)61687-0. View
2.
Amarilla A, de Almeida F, Jorge D, Alfonso H, de Castro-Jorge L, Nogueira N . Genetic diversity of the E protein of dengue type 3 virus. Virol J. 2009; 6:113. PMC: 2720943. DOI: 10.1186/1743-422X-6-113. View
3.
Mizuno Y, Kotaki A, Harada F, Tajima S, Kurane I, Takasaki T . Confirmation of dengue virus infection by detection of dengue virus type 1 genome in urine and saliva but not in plasma. Trans R Soc Trop Med Hyg. 2007; 101(7):738-9. DOI: 10.1016/j.trstmh.2007.02.007. View
4.
Johnson B, Russell B, Lanciotti R . Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay. J Clin Microbiol. 2005; 43(10):4977-83. PMC: 1248506. DOI: 10.1128/JCM.43.10.4977-4983.2005. View
5.
Vazquez S, Cabezas S, Perez A, Pupo M, Ruiz D, Calzada N . Kinetics of antibodies in sera, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infections. Int J Infect Dis. 2006; 11(3):256-62. DOI: 10.1016/j.ijid.2006.05.005. View