Aedes Mosquitoes Acquire and Transmit Zika Virus by Breeding in Contaminated Aquatic Environments

Overview

Journal Nat Commun

Specialty Biology

Date 2019 Mar 24

PMID 30902991

Citations 24

Authors

Senyan Du

Yang Liu

Jianying Liu

Jie Zhao

Clara Champagne

Liangqin Tong

Renli Zhang

Fuchun Zhang

Cheng-Feng Qin

Ping Ma

Chun-Hong Chen

Guodong Liang

Qiyong Liu

Pei-Yong Shi

Bernard Cazelles

Penghua Wang

Huaiyu Tian

Gong Cheng

Affiliations

Soon will be listed here.

Abstract

Zika virus (ZIKV) is a mosquito-borne flavivirus that predominantly circulates between humans and Aedes mosquitoes. Clinical studies have shown that Zika viruria in patients persists for an extended period, and results in infectious virions being excreted. Here, we demonstrate that Aedes mosquitoes are permissive to ZIKV infection when breeding in urine or sewage containing low concentrations of ZIKV. Mosquito larvae and pupae, including from field Aedes aegypti can acquire ZIKV from contaminated aquatic systems, resulting in ZIKV infection of adult females. Adult mosquitoes can transmit infectious virions to susceptible type I/II interferon receptor-deficient (ifnagr-/-) C57BL/6 (AG6) mice. Furthermore, ZIKV viruria from infected AG6 mice can causes mosquito infection during the aquatic life stages. Our studies suggest that infectious urine could be a natural ZIKV source, which is potentially transmissible to mosquitoes when breeding in an aquatic environment.

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References

Dupont-Rouzeyrol M, Biron A, OConnor O, Huguon E, Descloux E . Infectious Zika viral particles in breastmilk. Lancet. 2016; 387(10023):1051. DOI: 10.1016/S0140-6736(16)00624-3. View

Rose C, Parker A, Jefferson B, Cartmell E . The Characterization of Feces and Urine: A Review of the Literature to Inform Advanced Treatment Technology. Crit Rev Environ Sci Technol. 2015; 45(17):1827-1879. PMC: 4500995. DOI: 10.1080/10643389.2014.1000761. View

Marchette N, Garcia R, Rudnick A . Isolation of Zika virus from Aedes aegypti mosquitoes in Malaysia. Am J Trop Med Hyg. 1969; 18(3):411-5. DOI: 10.4269/ajtmh.1969.18.411. View

Foy B, Kobylinski K, Chilson Foy J, Blitvich B, da Rosa A, Haddow A . Probable non-vector-borne transmission of Zika virus, Colorado, USA. Emerg Infect Dis. 2011; 17(5):880-2. PMC: 3321795. DOI: 10.3201/eid1705.101939. View

Robey I, Nesbit L . Investigating mechanisms of alkalinization for reducing primary breast tumor invasion. Biomed Res Int. 2013; 2013:485196. PMC: 3722989. DOI: 10.1155/2013/485196. View

Pompon J, Morales-Vargas R, Manuel M, Tan C, Vial T, Tan J . A Zika virus from America is more efficiently transmitted than an Asian virus by Aedes aegypti mosquitoes from Asia. Sci Rep. 2017; 7(1):1215. PMC: 5430906. DOI: 10.1038/s41598-017-01282-6. View

Heinz F, Stiasny K, Holzmann H, Allison S, Mandl C, Kunz C . Structural changes and functional control of the tick-borne encephalitis virus glycoprotein E by the heterodimeric association with protein prM. Virology. 1994; 198(1):109-17. DOI: 10.1006/viro.1994.1013. View

Bai F, Town T, Pradhan D, Cox J, Ashish , Ledizet M . Antiviral peptides targeting the west nile virus envelope protein. J Virol. 2006; 81(4):2047-55. PMC: 1797586. DOI: 10.1128/JVI.01840-06. View

Burke R, Barrera R, Lewis M, Kluchinsky T, Claborn D . Septic tanks as larval habitats for the mosquitoes Aedes aegypti and Culex quinquefasciatus in Playa-Playita, Puerto Rico. Med Vet Entomol. 2010; 24(2):117-23. DOI: 10.1111/j.1365-2915.2010.00864.x. View

10.

Musso D, Roche C, Robin E, Nhan T, Teissier A, Cao-Lormeau V . Potential sexual transmission of Zika virus. Emerg Infect Dis. 2015; 21(2):359-61. PMC: 4313657. DOI: 10.3201/eid2102.141363. View

11.

Bonaldo M, Ribeiro I, Lima N, Dos Santos A, Menezes L, da Cruz S . Isolation of Infective Zika Virus from Urine and Saliva of Patients in Brazil. PLoS Negl Trop Dis. 2016; 10(6):e0004816. PMC: 4920388. DOI: 10.1371/journal.pntd.0004816. View

12.

Musso D, Roche C, Nhan T, Robin E, Teissier A, Cao-Lormeau V . Detection of Zika virus in saliva. J Clin Virol. 2015; 68:53-5. DOI: 10.1016/j.jcv.2015.04.021. View

13.

Robey I, Martin N . Bicarbonate and dichloroacetate: evaluating pH altering therapies in a mouse model for metastatic breast cancer. BMC Cancer. 2011; 11:235. PMC: 3125283. DOI: 10.1186/1471-2407-11-235. View

14.

Cheng G, Cox J, Wang P, Krishnan M, Dai J, Qian F . A C-type lectin collaborates with a CD45 phosphatase homolog to facilitate West Nile virus infection of mosquitoes. Cell. 2010; 142(5):714-25. PMC: 2954371. DOI: 10.1016/j.cell.2010.07.038. View

15.

Wang F, Lv Z, Wang J, Fu S, Zhang H, Wang Z . [Sequencing and analysis of the full coding sequence of Batai virus isolated in China]. Bing Du Xue Bao. 2009; 25(2):83-7. View

16.

Oehler E, Watrin L, Larre P, Leparc-Goffart I, Lastere S, Valour F . Zika virus infection complicated by Guillain-Barre syndrome--case report, French Polynesia, December 2013. Euro Surveill. 2014; 19(9). DOI: 10.2807/1560-7917.es2014.19.9.20720. View

17.

Liu J, Liu Y, Nie K, Du S, Qiu J, Pang X . Flavivirus NS1 protein in infected host sera enhances viral acquisition by mosquitoes. Nat Microbiol. 2016; 1(9):16087. PMC: 5003325. DOI: 10.1038/nmicrobiol.2016.87. View

18.

Chouin-Carneiro T, Vega-Rua A, Vazeille M, Yebakima A, Girod R, Goindin D . Differential Susceptibilities of Aedes aegypti and Aedes albopictus from the Americas to Zika Virus. PLoS Negl Trop Dis. 2016; 10(3):e0004543. PMC: 4777396. DOI: 10.1371/journal.pntd.0004543. View

19.

Fonseca K, Meatherall B, Zarra D, Drebot M, MacDonald J, Pabbaraju K . First case of Zika virus infection in a returning Canadian traveler. Am J Trop Med Hyg. 2014; 91(5):1035-8. PMC: 4228871. DOI: 10.4269/ajtmh.14-0151. View

20.

Lustig Y, Mendelson E, Paran N, Melamed S, Schwartz E . Detection of Zika virus RNA in whole blood of imported Zika virus disease cases up to 2 months after symptom onset, Israel, December 2015 to April 2016. Euro Surveill. 2016; 21(26). DOI: 10.2807/1560-7917.ES.2016.21.26.30269. View