Preferential Associations Between Co-regulated Genes Reveal a Transcriptional Interactome in Erythroid Cells

Overview

Journal Nat Genet

Specialty Genetics

Date 2009 Dec 17

PMID 20010836

Citations 416

Authors

Stefan Schoenfelder

Tom Sexton

Lyubomira Chakalova

Nathan F Cope

Alice Horton

Simon Andrews

Sreenivasulu Kurukuti

Jennifer A Mitchell

David Umlauf

Daniela S Dimitrova

Christopher H Eskiw

Yanquan Luo

Chia-Lin Wei

Yijun Ruan

James J Bieker

Peter Fraser

Affiliations

Soon will be listed here.

Abstract

The discovery of interchromosomal interactions in higher eukaryotes points to a functional interplay between genome architecture and gene expression, challenging the view of transcription as a one-dimensional process. However, the extent of interchromosomal interactions and the underlying mechanisms are unknown. Here we present the first genome-wide analysis of transcriptional interactions using the mouse globin genes in erythroid tissues. Our results show that the active globin genes associate with hundreds of other transcribed genes, revealing extensive and preferential intra- and interchromosomal transcription interactomes. We show that the transcription factor Klf1 mediates preferential co-associations of Klf1-regulated genes at a limited number of specialized transcription factories. Our results establish a new gene expression paradigm, implying that active co-regulated genes and their regulatory factors cooperate to create specialized nuclear hot spots optimized for efficient and coordinated transcriptional control.

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