Role of CAP350 in Centriolar Tubule Stability and Centriole Assembly

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2008 Dec 5

PMID 19052644

Citations 14

Authors

Mikael Le Clech

Affiliations

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Abstract

Background: Centrioles are microtubule-based cylindrical structures composed of nine triplet tubules and are required for the formation of the centrosome, flagella and cilia. Despite theirs importance, centriole biogenesis is poorly understood. Centrosome duplication is initiated at the G1/S transition by the sequential recruitment of a set of conserved proteins under the control of the kinase Plk4. Subsequently, the procentriole is assembled by the polymerization of centriolar tubules via an unknown mechanism involving several tubulin paralogs.

Methodology/principal Findings: Here, we developed a cellular assay to study centrosome duplication and procentriole stability based on its sensitivity to the microtubule-depolymerizing drug nocodazole. By using RNA interference experiments, we show that the stability of growing procentrioles is regulated by the microtubule-stabilizing protein CAP350, independently of hSAS-6 and CPAP which initiate procentriole growth. Furthermore, our analysis reveals the critical role of centriolar tubule stability for an efficient procentriole growth.

Conclusions/significance: CAP350 belongs to a new class of proteins which associate and stabilize centriolar tubules to control centriole duplication.

Citing Articles

Molecular basis promoting centriole triplet microtubule assembly.

Takeda Y, Chinen T, Honda S, Takatori S, Okuda S, Yamamoto S Nat Commun. 2024; 15(1):2216.

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Towards understanding centriole elimination.

Kalbfuss N, Gonczy P Open Biol. 2023; 13(11):230222.

PMID: 37963546 PMC: 10645514. DOI: 10.1098/rsob.230222.

The central scaffold protein CEP350 coordinates centriole length, stability, and maturation.

Karasu O, Neuner A, Atorino E, Pereira G, Schiebel E J Cell Biol. 2022; 221(12).

PMID: 36315013 PMC: 9623370. DOI: 10.1083/jcb.202203081.

Estrogens-Origin of Centrosome Defects in Human Cancer?.

Buhler M, Stolz A Cells. 2022; 11(3).

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A choreography of centrosomal mRNAs reveals a conserved localization mechanism involving active polysome transport.

Safieddine A, Coleno E, Salloum S, Imbert A, Traboulsi A, Kwon O Nat Commun. 2021; 12(1):1352.

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