The Chromosomal Association of Condensin II is Regulated by a Noncatalytic Function of PP2A

Overview

Journal Nat Struct Mol Biol

Specialties Cell Biology
Molecular Biology

Date 2009 Nov 17

PMID 19915589

Citations 32

Authors

Ai Takemoto

Kazuhiro Maeshima

Tsuyoshi Ikehara

Kazumitsu Yamaguchi

Akiko Murayama

Shihoko Imamura

Naoko Imamoto

Shigeyuki Yokoyama

Tatsuya Hirano

Yoshinori Watanabe

Fumio Hanaoka

Junn Yanagisawa

Keiji Kimura

Affiliations

Soon will be listed here.

Abstract

Mitotic chromosomal assembly in vertebrates is regulated by condensin I and condensin II, which work cooperatively but have different chromosomal localization profiles and make distinct mechanistic contributions to this process. We show here that protein phosphatase 2A (PP2A), which interacts with condensin II but not condensin I, plays an essential role in targeting condensin II to chromosomes. Unexpectedly, our data indicate that PP2A acts as a recruiter protein rather than a catalytic enzyme to target condensin II to chromosomes. This recruiting activity of PP2A was inhibited by okadaic acid, but not by fostriecin, even though both molecules strongly inhibited the catalytic activity of PP2A. Additionally, we found that the chromokinesin KIF4a is also targeted to chromosomes via the noncatalytic activity of PP2A. Thus, our studies reveal a previously unknown contribution of PP2A to chromosome assembly.

Citing Articles

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Paulson J, Hudson D, Cisneros-Soberanis F, Earnshaw W Semin Cell Dev Biol. 2021; 117:7-29.

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Protein phosphatases in chromatin structure and function.

Sales Gil R, Vagnarelli P Biochim Biophys Acta Mol Cell Res. 2018; 1866(1):90-101.

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Interdependency and phosphorylation of KIF4 and condensin I are essential for organization of chromosome scaffold.

Poonperm R, Takata H, Uchiyama S, Fukui K PLoS One. 2017; 12(8):e0183298.

PMID: 28817632 PMC: 5560531. DOI: 10.1371/journal.pone.0183298.

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