Reassociation with Beta 2-microglobulin is Necessary for Db Class I Major Histocompatibility Complex Binding of an Exogenous Influenza Peptide

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1991 Jan 1

PMID 1986378

Citations 13

Authors

K L Rock

S GAMBLE

L Rothstein

B Benacerraf

Affiliations

Soon will be listed here.

Abstract

A synthetic peptide corresponding to residues 365-380 of the influenza nucleoprotein (NP365-380) has been previously shown to associate with class I major histocompatibility complex-encoded molecules and to stimulate cytotoxic T lymphocytes [Townsend, A. R. M., Rothbard, J., Gotch, F. M., Bahadur, G., Wraith, D. & McMichael, A. J. (1986) Cell 44, 959-968]. We find that intact Db class I heterodimers on the cell surface are unreceptive to binding this antigen. However, NP365-380 readily associates with Db molecules on the plasma membrane in the presence of exogenous beta 2-microglobulin. In addition, there is a second pathway through which this peptide associates with class I molecules that requires energy and de novo protein synthesis. These findings have implications for maintaining the immunological identity of cells and for the use of peptides as vaccines for priming cytolytic T-cell immunity.

Citing Articles

Cross-presentation: dendritic cells and macrophages bite off more than they can chew!.

Brode S, Macary P Immunology. 2004; 112(3):345-51.

PMID: 15196201 PMC: 1782510. DOI: 10.1111/j.1365-2567.2004.01920.x.

Enhanced delivery of exogenous peptides into the class I antigen processing and presentation pathway.

de Haan L, Hearn A, Rivett A, Hirst T Infect Immun. 2002; 70(6):3249-58.

PMID: 12011020 PMC: 128024. DOI: 10.1128/IAI.70.6.3249-3258.2002.

An in vitro study of the dynamic features of the major histocompatibility complex class I complex relevant to its role as a versatile peptide-receptive molecule.

Horig H, Papadopoulos N, Vegh Z, Palmieri E, Angeletti R, Nathenson S Proc Natl Acad Sci U S A. 1998; 94(25):13826-31.

PMID: 9391112 PMC: 28392. DOI: 10.1073/pnas.94.25.13826.

Evidence for a conformational change in a class II major histocompatibility complex molecule occurring in the same pH range where antigen binding is enhanced.

Boniface J, Lyons D, Wettstein D, Allbritton N, Davis M J Exp Med. 1996; 183(1):119-26.

PMID: 8551214 PMC: 2192414. DOI: 10.1084/jem.183.1.119.

The class I-b molecule Qa-1 forms heterodimers with H-2Ld and a novel 50-kD glycoprotein encoded centromeric to I-E beta.

Wolf P, Cook R J Exp Med. 1995; 181(2):657-68.

PMID: 7836920 PMC: 2191872. DOI: 10.1084/jem.181.2.657.

References

Schwartz R . T-lymphocyte recognition of antigen in association with gene products of the major histocompatibility complex. Annu Rev Immunol. 1985; 3:237-61. DOI: 10.1146/annurev.iy.03.040185.001321. View

Allen P, Babbitt B, UNANUE E . T-cell recognition of lysozyme: the biochemical basis of presentation. Immunol Rev. 1987; 98:171-87. DOI: 10.1111/j.1600-065x.1987.tb00524.x. View

GAMMON G, Shastri N, Cogswell J, Wilbur S, Krzych U, Miller A . The choice of T-cell epitopes utilized on a protein antigen depends on multiple factors distant from, as well as at the determinant site. Immunol Rev. 1987; 98:53-73. DOI: 10.1111/j.1600-065x.1987.tb00519.x. View

Braciale T, Morrison L, Sweetser M, Sambrook J, Gething M, Braciale V . Antigen presentation pathways to class I and class II MHC-restricted T lymphocytes. Immunol Rev. 1987; 98:95-114. DOI: 10.1111/j.1600-065x.1987.tb00521.x. View

Bjorkman P, Saper M, Samraoui B, Bennett W, Strominger J, Wiley D . Structure of the human class I histocompatibility antigen, HLA-A2. Nature. 1987; 329(6139):506-12. DOI: 10.1038/329506a0. View

Yewdell J, Bennink J, Hosaka Y . Cells process exogenous proteins for recognition by cytotoxic T lymphocytes. Science. 1988; 239(4840):637-40. DOI: 10.1126/science.3257585. View

Brown J, Jardetzky T, Saper M, Samraoui B, Bjorkman P, Wiley D . A hypothetical model of the foreign antigen binding site of class II histocompatibility molecules. Nature. 1988; 332(6167):845-50. DOI: 10.1038/332845a0. View

Carbone F, Moore M, Sheil J, Bevan M . Induction of cytotoxic T lymphocytes by primary in vitro stimulation with peptides. J Exp Med. 1988; 167(6):1767-79. PMC: 2189675. DOI: 10.1084/jem.167.6.1767. View

Moore M, Carbone F, Bevan M . Introduction of soluble protein into the class I pathway of antigen processing and presentation. Cell. 1988; 54(6):777-85. DOI: 10.1016/s0092-8674(88)91043-4. View

10.

Townsend A, Elliott T, Cerundolo V, Foster L, Barber B, Tse A . Assembly of MHC class I molecules analyzed in vitro. Cell. 1990; 62(2):285-95. DOI: 10.1016/0092-8674(90)90366-m. View

11.

Rock K, Rothstein L, GAMBLE S . Generation of class I MHC-restricted T-T hybridomas. J Immunol. 1990; 145(3):804-11. View

12.

Ljunggren H, Stam N, Ohlen C, Neefjes J, Hoglund P, Heemels M . Empty MHC class I molecules come out in the cold. Nature. 1990; 346(6283):476-80. DOI: 10.1038/346476a0. View

13.

Rock K, GAMBLE S, Rothstein L . Presentation of exogenous antigen with class I major histocompatibility complex molecules. Science. 1990; 249(4971):918-21. DOI: 10.1126/science.2392683. View

14.

Perkins D, Lai M, Smith J, Gefter M . Identical peptides recognized by MHC class I- and II-restricted T cells. J Exp Med. 1989; 170(1):279-89. PMC: 2189383. DOI: 10.1084/jem.170.1.279. View

15.

Townsend A, Bodmer H . Antigen recognition by class I-restricted T lymphocytes. Annu Rev Immunol. 1989; 7:601-24. DOI: 10.1146/annurev.iy.07.040189.003125. View

16.

Rock K, Rothstein L, Gamble S, Benacerraf B . Reassociation with beta 2-microglobulin is necessary for Kb class I major histocompatibility complex binding of exogenous peptides. Proc Natl Acad Sci U S A. 1990; 87(19):7517-21. PMC: 54778. DOI: 10.1073/pnas.87.19.7517. View

17.

Townsend A, Ohlen C, Bastin J, Ljunggren H, Foster L, Karre K . Association of class I major histocompatibility heavy and light chains induced by viral peptides. Nature. 1989; 340(6233):443-8. DOI: 10.1038/340443a0. View

18.

Sweetser M, Morrison L, Braciale V, Braciale T . Recognition of pre-processed endogenous antigen by class I but not class II MHC-restricted T cells. Nature. 1989; 342(6246):180-2. DOI: 10.1038/342180a0. View

19.

Bevan M . Immunology. Stimulating killer cells. Nature. 1989; 342(6249):478-9. DOI: 10.1038/342478a0. View

20.

Hyafil F, Strominger J . Dissociation and exchange of the beta 2-microglobulin subunit of HLA-A and HLA-B antigens. Proc Natl Acad Sci U S A. 1979; 76(11):5834-8. PMC: 411746. DOI: 10.1073/pnas.76.11.5834. View