TSLP Production by Epithelial Cells Exposed to Immunodeficiency Virus Triggers DC-mediated Mucosal Infection of CD4+ T Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2009 Oct 7

PMID 19805372

Citations 32

Authors

Danielle Fontenot

Hong He

Shino Hanabuchi

Pramod N Nehete

Minying Zhang

Mikyoung Chang

Bharti Nehete

Yui-Hsi Wang

Yi-Hong Wang

Zhong-Min Ma

Hai-Chon Lee

Steven F Ziegler

Amy N Courtney

Christopher J Miller

Shao-Cong Sun

Yong-Jun Liu

K Jagannadha Sastry

Affiliations

Soon will be listed here.

Abstract

Mucosal dendritic cells have been implicated in the capture, storage, and transmission of HIV to CD4(+) T cells as well as in the promotion of HIV replication in activated CD4(+) T cells during the cognate T-cell and DC interaction. We report that HIV induces human genital mucosal epithelial cells to produce thymic stromal lymphopoietin (TSLP) via activation of the NFkappaB signaling pathway. The TSLP secreted by HIV exposed epithelial cells activated DC, which promoted proliferation and HIV-1 replication of co-cultured autologous CD4(+) T cells. In rhesus macaques, we observed dramatic increases in TSLP expression concurrent with an increase in viral replication in the vaginal tissues within the first 2 weeks after vaginal SIV exposure. These data suggest that HIV-mediated TSLP production by mucosal epithelial cells is a critical trigger for DC-mediated amplification of HIV-infection in activated CD4(+) T cells. The cross talk between mucosal epithelial cells and DC, mediated by HIV-induced TSLP, may be an important mechanism for the high rate of HIV infection in women through the vaginal mucosa.

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