Comparative Infection Efficiency of Porcine Reproductive and Respiratory Syndrome Virus Field Isolates on MA104 Cells and Porcine Alveolar Macrophages

Overview

Journal Can J Vet Res

Specialty Veterinary Medicine

Date 2009 Oct 2

PMID 19794892

Citations 12

Authors

Martha Fuentes de Abin

Gordon Spronk

Mark Wagner

Mark Fitzsimmons

Juan E Abrahante

Michael P Murtaugh

Affiliations

Soon will be listed here.

Abstract

Isolation of Porcine reproductive and respiratory syndrome virus (PRRSV) on MA104 or MARC-145 cells is frequently used in PRRS diagnosis. However, the ability of recent field isolates to grow on these established simian cell lines has not been determined. The aim of this study was to characterize the growth of PRRSV field isolates on primary porcine alveolar macrophages (PAMs) and MA104 cells in comparison with the growth of the laboratory-adapted strain VR-2332. A cytopathic effect was observed in 70% of serum samples after 1 passage on PAMs and was verified by immunofluorescent staining or reverse transcriptase-polymerase chain reaction. Field isolate growth was observed on MA104 cells for only 1 of 50 serum samples after 14 d. Strain VR-2332 grew readily in MA104 cells [maximum titer, 10(7) TCID(50) (median tissue culture infective dose) per milliliter at 30 h] but not in PAMs (10(2) TCID(50)/mL at 72 h). These results show that PAMs are superior to simian cells for diagnostic isolation of current field PRRSV strains.

Citing Articles

Research Progress on the GP3 Protein of Porcine Reproductive and Respiratory Syndrome Virus.

Lv C, Yang Z, Lan X, Liang F, Kong W, Wang R Animals (Basel). 2025; 15(3).

PMID: 39943200 PMC: 11815881. DOI: 10.3390/ani15030430.

Development of a one-step reverse transcription-quantitative polymerase chain reaction assay for the detection of porcine reproductive and respiratory syndrome virus.

Chae H, Roh H, Jo Y, Kim W, Chae J, Shin S PLoS One. 2023; 18(10):e0293042.

PMID: 37844073 PMC: 10578580. DOI: 10.1371/journal.pone.0293042.

Influence of PRRSV-1 vaccination and infection on mononuclear immune cells at the maternal-fetal interface.

Stas M, Kreutzmann H, Stadler J, Sassu E, Mair K, Koch M Front Immunol. 2022; 13:1055048.

PMID: 36426366 PMC: 9679432. DOI: 10.3389/fimmu.2022.1055048.

Comparison of Primary Virus Isolation in Pulmonary Alveolar Macrophages and Four Different Continuous Cell Lines for Type 1 and Type 2 Porcine Reproductive and Respiratory Syndrome Virus.

Xie J, Vereecke N, Theuns S, Oh D, Vanderheijden N, Trus I Vaccines (Basel). 2021; 9(6).

PMID: 34205087 PMC: 8229515. DOI: 10.3390/vaccines9060594.

Comparison of ZMAC and MARC-145 Cell Lines for Improving Porcine Reproductive and Respiratory Syndrome Virus Isolation from Clinical Samples.

Yim-Im W, Huang H, Park J, Wang C, Calzada G, Gauger P J Clin Microbiol. 2020; 59(3).

PMID: 33268540 PMC: 8106717. DOI: 10.1128/JCM.01757-20.

References

Ait-Ali T, Wilson A, Westcott D, Clapperton M, Waterfall M, Mellencamp M . Innate immune responses to replication of porcine reproductive and respiratory syndrome virus in isolated Swine alveolar macrophages. Viral Immunol. 2007; 20(1):105-18. DOI: 10.1089/vim.2006.0078. View

Mayer P, Lam C . Porcine alveolar macrophages, isolation, morphological and functional characteristics. Zentralbl Veterinarmed A. 1984; 31(1):59-71. DOI: 10.1111/j.1439-0442.1984.tb01262.x. View

Valicek L, Psikal I, Smid B, Rodak L, Kubalikova R, Kosinova E . Isolation and identification of porcine reproductive and respiratory syndrome virus in cell cultures. Vet Med (Praha). 1998; 42(10):281-7. View

Mengeling W, Vorwald A, Lager K, Brockmeier S . Diagnosis of porcine reproductive and respiratory syndrome using infected alveolar macrophages collected from live pigs. Vet Microbiol. 1996; 49(1-2):105-15. DOI: 10.1016/0378-1135(95)00173-5. View

Magar R, Robinson Y, Dubuc C, Larochelle R . Isolation and experimental oral transmission in pigs of a porcine reproductive and respiratory syndrome virus isolate. Adv Exp Med Biol. 1995; 380:139-44. DOI: 10.1007/978-1-4615-1899-0_23. View

Mengeling W, Lager K, Vorwald A . Alveolar macrophages as a diagnostic sample for detecting natural infection of pigs with porcine reproductive and respiratory syndrome virus. J Vet Diagn Invest. 1996; 8(2):238-40. DOI: 10.1177/104063879600800215. View

Bustos M, Nogal M, Revilla Y, Carrascosa A . Plaque assay for African swine fever virus on swine macrophages. Arch Virol. 2002; 147(7):1453-9. DOI: 10.1007/s00705-002-0814-6. View

Fuentes M, Pijoan C . Phagocytosis and intracellular killing of Pasteurella multocida by porcine alveolar macrophages after infection with pseudorabies virus. Vet Immunol Immunopathol. 1986; 13(1-2):165-72. DOI: 10.1016/0165-2427(86)90057-7. View

Albina E . [Porcine reproductive and respiratory syndrome: ten years of experience (1986-1996) with this undesirable viral infection]. Vet Res. 1997; 28(4):305-52. View

10.

Benfield D, Nelson E, Collins J, Harris L, Goyal S, Robison D . Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332). J Vet Diagn Invest. 1992; 4(2):127-33. DOI: 10.1177/104063879200400202. View

11.

Rossow K . Porcine reproductive and respiratory syndrome. Vet Pathol. 1998; 35(1):1-20. DOI: 10.1177/030098589803500101. View

12.

Calvert J, Slade D, Shields S, Jolie R, Mannan R, Ankenbauer R . CD163 expression confers susceptibility to porcine reproductive and respiratory syndrome viruses. J Virol. 2007; 81(14):7371-9. PMC: 1933360. DOI: 10.1128/JVI.00513-07. View

13.

Meulenberg J . PRRSV, the virus. Vet Res. 2000; 31(1):11-21. DOI: 10.1051/vetres:2000103. View

14.

Bautista E, Goyal S, Yoon I, Joo H, Collins J . Comparison of porcine alveolar macrophages and CL 2621 for the detection of porcine reproductive and respiratory syndrome (PRRS) virus and anti-PRRS antibody. J Vet Diagn Invest. 1993; 5(2):163-5. DOI: 10.1177/104063879300500204. View

15.

Baron T, Albina E, Leforban Y, Madec F, Guilmoto H, Plana Duran J . Report on the first outbreaks of the porcine reproductive and respiratory syndrome (PRRS) in France. Diagnosis and viral isolation. Ann Rech Vet. 1992; 23(2):161-6. View

16.

Key K, Guenette D, Yoon K, Halbur P, Toth T, Meng X . Development of a heteroduplex mobility assay to identify field isolates of porcine reproductive and respiratory syndrome virus with nucleotide sequences closely related to those of modified live-attenuated vaccines. J Clin Microbiol. 2003; 41(6):2433-9. PMC: 156521. DOI: 10.1128/JCM.41.6.2433-2439.2003. View

17.

Schurrer J, Dee S, Moon R, Murtaugh M, Finnegan C, Deen J . Retention of ingested porcine reproductive and respiratory syndrome virus in houseflies. Am J Vet Res. 2005; 66(9):1517-25. DOI: 10.2460/ajvr.2005.66.1517. View

18.

Kleiboeker S, Schommer S, Lee S, Watkins S, Chittick W, Polson D . Simultaneous detection of North American and European porcine reproductive and respiratory syndrome virus using real-time quantitative reverse transcriptase-PCR. J Vet Diagn Invest. 2005; 17(2):165-70. DOI: 10.1177/104063870501700211. View