» Articles » PMID: 19789178

Centrosomal Localization of Diversin and Its Relevance to Wnt Signaling

Overview
Journal J Cell Sci
Specialty Cell Biology
Date 2009 Oct 1
PMID 19789178
Citations 32
Authors
Affiliations
Soon will be listed here.
Abstract

Wnt pathways regulate many developmental processes, including cell-fate specification, cell polarity, and cell movements during morphogenesis. The subcellular distribution of pathway mediators in specific cellular compartments might be crucial for the selection of pathway targets and signaling specificity. We find that the ankyrin-repeat protein Diversin, which functions in different Wnt signaling branches, localizes to the centrosome in Xenopus ectoderm and mammalian cells. Upon stimulation with Wnt ligands, the centrosomal distribution of Diversin is transformed into punctate cortical localization. Also, Diversin was recruited by Frizzled receptors to non-homogeneous Dishevelled-containing cortical patches. Importantly, Diversin deletion constructs, which did not localize to the centrosome, failed to efficiently antagonize Wnt signaling. Furthermore, a C-terminal construct that interfered with Diversin localization inhibited Diversin-mediated beta-catenin degradation. These observations suggest that the centrosomal localization of Diversin is crucial for its function in Wnt signaling.

Citing Articles

Bystanders or active players: the role of extra centrosomes as signaling hubs.

Purkerson M, Amend S, Pienta K Cancer Metastasis Rev. 2024; 44(1):1.

PMID: 39570514 PMC: 11582193. DOI: 10.1007/s10555-024-10224-4.


Aberrant centrosome biogenesis disrupts nephron and collecting duct progenitor growth and fate resulting in fibrocystic kidney disease.

Cheng T, Agwu C, Shim K, Wang B, Jain S, Mahjoub M Development. 2023; 150(24).

PMID: 37982452 PMC: 10753588. DOI: 10.1242/dev.201976.


Genomic hallmarks and therapeutic implications of G0 cell cycle arrest in cancer.

Wiecek A, Cutty S, Kornai D, Parreno-Centeno M, Gourmet L, Tagliazucchi G Genome Biol. 2023; 24(1):128.

PMID: 37221612 PMC: 10204193. DOI: 10.1186/s13059-023-02963-4.


Keep Calm and Carry on with Extra Centrosomes.

Kalkan B, Ozcan S, Quintyne N, Reed S, Acilan C Cancers (Basel). 2022; 14(2).

PMID: 35053604 PMC: 8774008. DOI: 10.3390/cancers14020442.


Pan-cancer analyses demonstrate that ANKRD6 is associated with a poor prognosis and correlates with M2 macrophage infiltration in colon cancer.

Bai R, Wu D, Shi Z, Hu W, Li J, Chen Y Chin J Cancer Res. 2021; 33(1):93-102.

PMID: 33707932 PMC: 7941690. DOI: 10.21147/j.issn.1000-9604.2021.01.10.


References
1.
Sokol S . A role for Wnts in morpho-genesis and tissue polarity. Nat Cell Biol. 2000; 2(7):E124-5. DOI: 10.1038/35017136. View

2.
Eggenschwiler J, Anderson K . Cilia and developmental signaling. Annu Rev Cell Dev Biol. 2007; 23:345-73. PMC: 2094042. DOI: 10.1146/annurev.cellbio.23.090506.123249. View

3.
Wolda S, Moody C, Moon R . Overlapping expression of Xwnt-3A and Xwnt-1 in neural tissue of Xenopus laevis embryos. Dev Biol. 1993; 155(1):46-57. DOI: 10.1006/dbio.1993.1005. View

4.
Tree D, Shulman J, Rousset R, Scott M, Gubb D, Axelrod J . Prickle mediates feedback amplification to generate asymmetric planar cell polarity signaling. Cell. 2002; 109(3):371-81. DOI: 10.1016/s0092-8674(02)00715-8. View

5.
Ciruna B, Jenny A, Lee D, Mlodzik M, Schier A . Planar cell polarity signalling couples cell division and morphogenesis during neurulation. Nature. 2006; 439(7073):220-4. PMC: 1417047. DOI: 10.1038/nature04375. View