Leukotriene-E4 in Human Urine: Comparison of On-line Purification and Liquid Chromatography-tandem Mass Spectrometry to Affinity Purification Followed by Enzyme Immunoassay

Overview

Journal J Chromatogr B Analyt Technol Biomed Life Sci

Publisher Elsevier

Specialties Biomedical Engineering
Chemistry

Date 2009 Sep 4

PMID 19726242

Citations 20

Authors

Michael Armstrong

Andrew H Liu

Ronald Harbeck

Rick Reisdorph

Nathan Rabinovitch

Nichole Reisdorph

Affiliations

Soon will be listed here.

Abstract

A new analytical method suitable for high throughput measurements of LTE(4) in human urine is described. The methodology utilizes on-line enrichment and liquid chromatography/tandem mass spectrometry (LC/MS/MS). The novel LC/MS/MS method is rapid, linear from 5 to 500pg/ml in spiked urine samples of both healthy and asthmatic subjects and more accurate and precise than enzyme immunoassay (EIA) and previous LC/MS/MS methods. Results from sample integrity experiments and preliminary values of urinary LTE(4) from healthy adults and children are reported.

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