Stable-isotope Dilution LC–MS for Quantitative Biomarker Analysis

Overview

Journal Bioanalysis

Specialty Chemistry

Date 2010 Mar 31

PMID 20352077

Citations 78

Authors

Eugene Ciccimaro

Ian A Blair

Affiliations

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Abstract

The ability to conduct validated analyses of biomarkers is critically important in order to establish the sensitivity and selectivity of the biomarker in identifying a particular disease. The use of stable-isotope dilution (SID) methodology in combination with LC–MS/MS provides the highest possible analytical specificity for quantitative determinations. This methodology is now widely used in the discovery and validation of putative exposure and disease biomarkers. This review will describe the application of SID LC–MS methodology for the analysis of small-molecule and protein biomarkers. It will also discuss potential future directions for the use of this methodology for rigorous biomarker analysis.

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