Functional Significance of Tapasin Membrane Association and Disulfide Linkage to ERp57 in MHC Class I Presentation

Overview

Journal Eur J Immunol

Specialty Allergy & Immunology

Date 2009 Aug 25

PMID 19701894

Citations 11

Authors

Nathalie Vigneron

David R Peaper

Ralf M Leonhardt

Peter Cresswell

Affiliations

Soon will be listed here.

Abstract

Tapasin is disulfide linked to ERp57 within the peptide loading complex. In cell-free assays, a soluble variant of the tapasin/ERp57 dimer recruits MHC class I molecules and promotes peptide binding to them, whereas soluble tapasin alone does not. Here we show that within cells, tapasin conjugation with ERp57 is as critical as its integration into the membrane for efficient MHC class I assembly, surface expression, and Ag presentation to CD8(+) T cells. Elimination of both of these properties severely compromises tapasin function, in keeping with predictions from in vitro studies.

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