Expression of the PsbDII Gene in Synechococcus Sp. Strain PCC 7942 Requires Sequences Downstream of the Transcription Start Site

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 Dec 1

PMID 1938947

Citations 62

Authors

S A Bustos

S S Golden

Affiliations

Soon will be listed here.

Abstract

The psbDI and psbDII genes in Synechococcus sp. strain PCC 7942 encode the D2 polypeptide, an essential component of the photosystem II reaction center. Previous studies have demonstrated that transcripts from psbDII, but not psbDI, increase in response to high light intensity. Soluble proteins from Synechococcus cells shifted to high light were found to have affinity for DNA sequences upstream from the psbDII coding region. DNA mobility-shift and copper-phenanthroline footprinting assays of a 258-bp fragment revealed three distinct DNA-protein complexes that mapped to the untranslated leader region between +11 and +84. Deletion of the upstream flanking region to -42 had no effect on the expression of a psbDII-lacZ reporter gene or its induction by light, whereas a promoterless construct supported only minimal background levels of beta-galactosidase. A 4-bp deletion within the first protected region of the footprint decreased the beta-galactosidase activity to approximately 2% of that of the undeleted control, but gene expression remained responsive to light. Deletion of the three protected regions completely abolished both gene expression and light induction. These results suggest that the psbDII gene requires elements within the untranslated leader region for efficient gene expression, one of which may be involved in regulation by light.

Citing Articles

Bacteria can anticipate the seasons: Photoperiodism in cyanobacteria.

Jabbur M, Bratton B, Johnson C Science. 2024; 385(6713):1105-1111.

PMID: 39236161 PMC: 11473183. DOI: 10.1126/science.ado8588.

Clocking out and letting go to unleash green biotech applications in a photosynthetic host.

Xu Y, Jabbur M, Mori T, Young J, Johnson C Proc Natl Acad Sci U S A. 2024; 121(21):e2318690121.

PMID: 38739791 PMC: 11127020. DOI: 10.1073/pnas.2318690121.

The GGDEF protein Dgc2 suppresses both motility and biofilm formation in the filamentous cyanobacterium .

Toida K, Kushida W, Yamamoto H, Yamamoto K, Ishii K, Uesaka K Microbiol Spectr. 2023; :e0483722.

PMID: 37655901 PMC: 10581220. DOI: 10.1128/spectrum.04837-22.

: A model system for expanding the study of cyanobacterial circadian rhythms.

Zhao C, Xu Y, Wang B, Johnson C Front Physiol. 2023; 13:1085959.

PMID: 36685199 PMC: 9846126. DOI: 10.3389/fphys.2022.1085959.

KidA, a multi-PAS domain protein, tunes the period of the cyanobacterial circadian oscillator.

Kim S, Chi C, Pattanayak G, Dinner A, Rust M Proc Natl Acad Sci U S A. 2022; 119(37):e2202426119.

PMID: 36067319 PMC: 9478674. DOI: 10.1073/pnas.2202426119.

References

Golden S, Cho D, Nalty M . Two functional psbD genes in the cyanobacterium Synechococcus sp. strain PCC 7942. J Bacteriol. 1989; 171(9):4707-13. PMC: 210270. DOI: 10.1128/jb.171.9.4707-4713.1989. View

Easton A, Kushner S . Transcription of the uvrD gene of Escherichia coli is controlled by the lexA repressor and by attenuation. Nucleic Acids Res. 1983; 11(24):8625-40. PMC: 326612. DOI: 10.1093/nar/11.24.8625. View

van den Elzen P, Maat J, Walters H, Veltkamp E, Nijkamp H . The nucleotide sequence of the bacteriocin promoters of plasmids Clo DF13 and Co1 E1: role of lexA repressor and cAMP in the regulation of promoter activity. Nucleic Acids Res. 1982; 10(6):1913-28. PMC: 320580. DOI: 10.1093/nar/10.6.1913. View

Morden C, Golden S . Sequence analysis and phylogenetic reconstruction of the genes encoding the large and small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase from the chlorophyll b-containing prokaryote Prochlorothrix hollandica. J Mol Evol. 1991; 32(5):379-95. DOI: 10.1007/BF02101278. View

Ebina Y, Kishi F, Miki T, KAGAMIYAMA H, Nakazawa T, Nakazawa A . The nucleotide sequence surrounding the promoter region of colicin E1 gene. Gene. 1981; 15(2-3):119-26. DOI: 10.1016/0378-1119(81)90121-9. View

Fisher R, Long S . DNA footprint analysis of the transcriptional activator proteins NodD1 and NodD3 on inducible nod gene promoters. J Bacteriol. 1989; 171(10):5492-502. PMC: 210389. DOI: 10.1128/jb.171.10.5492-5502.1989. View

Pfeifer K, Prezant T, Guarente L . Yeast HAP1 activator binds to two upstream activation sites of different sequence. Cell. 1987; 49(1):19-27. DOI: 10.1016/0092-8674(87)90751-3. View

Nanba O, Satoh K . Isolation of a photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559. Proc Natl Acad Sci U S A. 1987; 84(1):109-12. PMC: 304151. DOI: 10.1073/pnas.84.1.109. View

Downard J, Kim S, Kil K . Localization of the cis-acting regulatory DNA sequences of the Myxococcus xanthus tps and ops genes. J Bacteriol. 1988; 170(10):4931-8. PMC: 211540. DOI: 10.1128/jb.170.10.4931-4938.1988. View

10.

Raibaud O, Schwartz M . Positive control of transcription initiation in bacteria. Annu Rev Genet. 1984; 18:173-206. DOI: 10.1146/annurev.ge.18.120184.001133. View

11.

Soberon X, Covarrubias L, Bolivar F . Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene. 1980; 9(3-4):287-305. DOI: 10.1016/0378-1119(90)90328-o. View

12.

Kil K, Brown G, Downard J . A segment of Myxococcus xanthus ops DNA functions as an upstream activation site for tps gene transcription. J Bacteriol. 1990; 172(6):3081-8. PMC: 209111. DOI: 10.1128/jb.172.6.3081-3088.1990. View

13.

Ninfa A, Reitzer L, MAGASANIK B . Initiation of transcription at the bacterial glnAp2 promoter by purified E. coli components is facilitated by enhancers. Cell. 1987; 50(7):1039-46. DOI: 10.1016/0092-8674(87)90170-x. View

14.

Nakatani Y, Horikoshi M, Brenner M, Yamamoto T, Besnard F, Roeder R . A downstream initiation element required for efficient TATA box binding and in vitro function of TFIID. Nature. 1990; 348(6296):86-8. DOI: 10.1038/348086a0. View

15.

Golden S, Brusslan J, Haselkorn R . Expression of a family of psbA genes encoding a photosystem II polypeptide in the cyanobacterium Anacystis nidulans R2. EMBO J. 1986; 5(11):2789-98. PMC: 1167224. DOI: 10.1002/j.1460-2075.1986.tb04569.x. View

16.

Mizusawa S, Court D, Gottesman S . Transcription of the sulA gene and repression by LexA. J Mol Biol. 1983; 171(3):337-43. DOI: 10.1016/0022-2836(83)90097-9. View

17.

Holschuh K, Bottomley W, Whitfeld P . Structure of the spinach chloroplast genes for the D2 and 44 kd reaction-centre proteins of photosystem II and for tRNASer (UGA). Nucleic Acids Res. 1984; 12(23):8819-34. PMC: 320421. DOI: 10.1093/nar/12.23.8819. View

18.

Bustos S, Schaefer M, Golden S . Different and rapid responses of four cyanobacterial psbA transcripts to changes in light intensity. J Bacteriol. 1990; 172(4):1998-2004. PMC: 208697. DOI: 10.1128/jb.172.4.1998-2004.1990. View

19.

Kuwabara M, Sigman D . Footprinting DNA-protein complexes in situ following gel retardation assays using 1,10-phenanthroline-copper ion: Escherichia coli RNA polymerase-lac promoter complexes. Biochemistry. 1987; 26(23):7234-8. DOI: 10.1021/bi00397a006. View

20.

Moura-Neto R, Dudov K, PERRY R . An element downstream of the cap site is required for transcription of the gene encoding mouse ribosomal protein L32. Proc Natl Acad Sci U S A. 1989; 86(11):3997-4001. PMC: 287375. DOI: 10.1073/pnas.86.11.3997. View