Alpha 2.0
Login Register
» Articles » PMID: 16593792

Isolation of a Photosystem II Reaction Center Consisting of D-1 and D-2 Polypeptides and Cytochrome B-559

Overview
Journal Proc Natl Acad Sci U S A
Specialty Science
Date 1987 Jan 1
PMID 16593792
Citations 284
Authors
O Nanba
K Satoh
Affiliations
Soon will be listed here.
Abstract

A photosystem II reaction center complex consisting of D-1 and D-2 polypeptides and cytochrome b-559 was isolated from spinach grana thylakoids, treated with 4% (wt/vol) Triton X-100, by ion-exchange chromatography using DEAE-Toyopearl 650S. The isolated complex appears to contain five chlorophyll a, two pheophytin a, one beta-carotene, and one or two cytochrome b-559 heme(s) (molar ratio) and exhibits a reversible absorbance change attributable to the photochemical accumulation of reduced pheophytin typical for the intermediary electron acceptor of photosystem II reaction center. These results strongly suggest that the site of primary charge separation in photosystem II is located on the heterodimer composed of D-1 and D-2 subunits.

Citing Articles

RESISTANCE TO PHYTOPHTHORA1 promotes cytochrome b559 formation during early photosystem II biogenesis in Arabidopsis.

Che L, Ruan J, Xin Q, Zhang L, Gao F, Cai L Plant Cell. 2024; 36(10):4143-4167.

PMID: 38963884 PMC: 11449094. DOI: 10.1093/plcell/koae196.

Revelations on photosystem II, thermoluminescence, and artificial photosynthesis: a retrospective of Govindjee from fundamentals to applications.

Veena M, Sameena P, Sarath N, Noble L, Aswathi K, Amritha M Physiol Mol Biol Plants. 2023; 29(9):1225-1238.

PMID: 38024954 PMC: 10678879. DOI: 10.1007/s12298-023-01373-x.

Charge separation in the photosystem II reaction center resolved by multispectral two-dimensional electronic spectroscopy.

Nguyen H, Song Y, Maret E, Silori Y, Willow R, Yocum C Sci Adv. 2023; 9(18):eade7190.

PMID: 37134172 PMC: 10156117. DOI: 10.1126/sciadv.ade7190.

Advances in the Understanding of the Lifecycle of Photosystem II.

Johnson V, Pakrasi H Microorganisms. 2022; 10(5).

PMID: 35630282 PMC: 9145668. DOI: 10.3390/microorganisms10050836.

Remembering James Barber (1940-2020).

Nixon P, Telfer A Photosynth Res. 2022; 153(1-2):1-20.

PMID: 35534741 PMC: 9522743. DOI: 10.1007/s11120-022-00919-6.

References
1.
Aoki K, Ideguchi T, Kakuno T, Yamashita J, Horio T . Intermolecular relations of the photosystem II complex in spinach chloroplasts as detected by immunochemical assay. J Biochem. 1986; 100(4):875-82. DOI: 10.1093/oxfordjournals.jbchem.a121800. View
2.
Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View
3.
Delepelaire P, Chua N . Lithium dodecyl sulfate/polyacrylamide gel electrophoresis of thylakoid membranes at 4 degrees C: Characterizations of two additional chlorophyll a-protein complexes. Proc Natl Acad Sci U S A. 1979; 76(1):111-5. PMC: 382886. DOI: 10.1073/pnas.76.1.111. View
4.
Deisenhofer J, Epp O, Miki K, Huber R, Michel H . X-ray structure analysis of a membrane protein complex. Electron density map at 3 A resolution and a model of the chromophores of the photosynthetic reaction center from Rhodopseudomonas viridis. J Mol Biol. 1984; 180(2):385-98. DOI: 10.1016/s0022-2836(84)80011-x. View
5.
Hind G, Nakatani H . Determination of the concentration and the redox potential o chloroplast cytochrome 559. Biochim Biophys Acta. 1970; 216(1):223-5. DOI: 10.1016/0005-2728(70)90174-x. View