Evidence for Interactions Between MotA and MotB, Torque-generating Elements of the Flagellar Motor of Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 Nov 1

PMID 1938906

Citations 65

Authors

B Stolz

H C Berg

Affiliations

Soon will be listed here.

Abstract

Cells that overexpress MotA (encoded on a plasmid derived from pBR322) grow slowly because of proton leakage. We have traced this defect to the coexpression of a fusion protein consisting of 60 amino acids from the N terminus of MotB and 50 amino acids specified by pBR322. Mutations within the N terminus, known to abolish function when present in full-length MotB, reversed the growth defect. Growth also was normal when MotA was coexpressed with wild-type MotB or with a series of MotB N-terminal fragments.

Citing Articles

Sodium-Dependent Conformational Change in Flagellar Stator Protein MotS from .

Takekawa N, Yamaguchi A, Nishiuchi K, Uehori M, Kinoshita M, Minamino T Biomolecules. 2025; 15(2).

PMID: 40001605 PMC: 11853200. DOI: 10.3390/biom15020302.

Overexpression of the flagellar motor protein MotB sensitizes Bacillus subtilis to aminoglycosides in a motility-independent manner.

Uneme M, Ishikawa K, Furuta K, Yamashita A, Kaito C PLoS One. 2024; 19(4):e0300634.

PMID: 38669243 PMC: 11051680. DOI: 10.1371/journal.pone.0300634.

Chemotactic response of p-nitrophenol degrading strain PNPG3 through phenotypic and genome sequence-based in silico studies.

Alam S, Saha P 3 Biotech. 2023; 13(12):408.

PMID: 37987023 PMC: 10657342. DOI: 10.1007/s13205-023-03809-3.

FlgV forms a flagellar motor ring that is required for optimal motility of Helicobacter pylori.

Botting J, Tachiyama S, Gibson K, Liu J, Starai V, Hoover T PLoS One. 2023; 18(11):e0287514.

PMID: 37976320 PMC: 10655999. DOI: 10.1371/journal.pone.0287514.

Control of the flagellation pattern in by FlhF and FlhG.

Gibson K, Botting J, Al-Otaibi N, Maitre K, Bergeron J, Starai V J Bacteriol. 2023; 205(9):e0011023.

PMID: 37655916 PMC: 10521351. DOI: 10.1128/jb.00110-23.

References

Armstrong J, Adler J . Location of genes for motility and chemotaxis on the Escherichia coli genetic map. J Bacteriol. 1969; 97(1):156-61. PMC: 249567. DOI: 10.1128/jb.97.1.156-161.1969. View

Jones C, Aizawa S . The bacterial flagellum and flagellar motor: structure, assembly and function. Adv Microb Physiol. 1991; 32:109-72. DOI: 10.1016/s0065-2911(08)60007-7. View

Silverman M, Matsumura P, Simon M . The identification of the mot gene product with Escherichia coli-lambda hybrids. Proc Natl Acad Sci U S A. 1976; 73(9):3126-30. PMC: 430953. DOI: 10.1073/pnas.73.9.3126. View

Silverman M, Simon M . Operon controlling motility and chemotoxis in E. coli. Nature. 1976; 264(5586):577-80. DOI: 10.1038/264577a0. View

PARKINSON J . Complementation analysis and deletion mapping of Escherichia coli mutants defective in chemotaxis. J Bacteriol. 1978; 135(1):45-53. PMC: 224763. DOI: 10.1128/jb.135.1.45-53.1978. View

Boyd A, Mandel G, Simon M . Integral membrane proteins required for bacterial motility and chemotaxis. Symp Soc Exp Biol. 1982; 35:123-37. View

Block S, Berg H . Successive incorporation of force-generating units in the bacterial rotary motor. Nature. 1984; 309(5967):470-2. DOI: 10.1038/309470a0. View

Dean G, Macnab R, Stader J, Matsumura P, BURKS C . Gene sequence and predicted amino acid sequence of the motA protein, a membrane-associated protein required for flagellar rotation in Escherichia coli. J Bacteriol. 1984; 159(3):991-9. PMC: 215758. DOI: 10.1128/jb.159.3.991-999.1984. View

Tabor S, Richardson C . A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci U S A. 1985; 82(4):1074-8. PMC: 397196. DOI: 10.1073/pnas.82.4.1074. View

10.

Yamaguchi S, Fujita H, Ishihara A, Aizawa S, Macnab R . Subdivision of flagellar genes of Salmonella typhimurium into regions responsible for assembly, rotation, and switching. J Bacteriol. 1986; 166(1):187-93. PMC: 214575. DOI: 10.1128/jb.166.1.187-193.1986. View

11.

Stader J, Matsumura P, Vacante D, Dean G, Macnab R . Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane. J Bacteriol. 1986; 166(1):244-52. PMC: 214583. DOI: 10.1128/jb.166.1.244-252.1986. View

12.

Yamaguchi S, Aizawa S, Kihara M, Isomura M, Jones C, Macnab R . Genetic evidence for a switching and energy-transducing complex in the flagellar motor of Salmonella typhimurium. J Bacteriol. 1986; 168(3):1172-9. PMC: 213619. DOI: 10.1128/jb.168.3.1172-1179.1986. View

13.

Chun S, PARKINSON J . Bacterial motility: membrane topology of the Escherichia coli MotB protein. Science. 1988; 239(4837):276-8. DOI: 10.1126/science.2447650. View

14.

Wilson M, Macnab R . Overproduction of the MotA protein of Escherichia coli and estimation of its wild-type level. J Bacteriol. 1988; 170(2):588-97. PMC: 210695. DOI: 10.1128/jb.170.2.588-597.1988. View

15.

Khan S, DAPICE M, Reese T . Effects of mot gene expression on the structure of the flagellar motor. J Mol Biol. 1988; 202(3):575-84. DOI: 10.1016/0022-2836(88)90287-2. View

16.

Macnab R, DeRosier D . Bacterial flagellar structure and function. Can J Microbiol. 1988; 34(4):442-51. DOI: 10.1139/m88-077. View

17.

Blair D, Berg H . Restoration of torque in defective flagellar motors. Science. 1988; 242(4886):1678-81. DOI: 10.1126/science.2849208. View

18.

Meister M, Caplan S, Berg H . Dynamics of a tightly coupled mechanism for flagellar rotation. Bacterial motility, chemiosmotic coupling, protonmotive force. Biophys J. 1989; 55(5):905-14. PMC: 1330527. DOI: 10.1016/S0006-3495(89)82889-9. View

19.

Blair D, Berg H . The MotA protein of E. coli is a proton-conducting component of the flagellar motor. Cell. 1990; 60(3):439-49. DOI: 10.1016/0092-8674(90)90595-6. View

20.

Wilson M, Macnab R . Co-overproduction and localization of the Escherichia coli motility proteins motA and motB. J Bacteriol. 1990; 172(7):3932-9. PMC: 213376. DOI: 10.1128/jb.172.7.3932-3939.1990. View