Ozone and Allergen Exposure During Postnatal Development Alters the Frequency and Airway Distribution of CD25+ Cells in Infant Rhesus Monkeys

Overview

Journal Toxicol Appl Pharmacol

Specialties Pharmacology
Toxicology

Date 2009 Apr 18

PMID 19371618

Citations 8

Authors

Lisa A Miller

Joan E Gerriets

Nancy K Tyler

Kristina Abel

Edward S Schelegle

Charles G Plopper

Dallas M Hyde

Affiliations

Soon will be listed here.

Abstract

The epidemiologic link between air pollutant exposure and asthma has been supported by experimental findings, but the mechanisms are not understood. In this study, we evaluated the impact of combined ozone and house dust mite (HDM) exposure on the immunophenotype of peripheral blood and airway lymphocytes from rhesus macaque monkeys during the postnatal period of development. Starting at 30 days of age, monkeys were exposed to 11 cycles of filtered air, ozone, HDM aerosol, or ozone+HDM aerosol. Each cycle consisted of ozone delivered at 0.5 ppm for 5 days (8 h/day), followed by 9 days of filtered air; animals received HDM aerosol during the last 3 days of each ozone exposure period. Between 2-3 months of age, animals co-exposed to ozone+HDM exhibited a decline in total circulating leukocyte numbers and increased total circulating lymphocyte frequency. At 3 months of age, blood CD4+/CD25+ lymphocytes were increased with ozone+HDM. At 6 months of age, CD4+/CD25+ and CD8+/CD25+ lymphocyte populations increased in both blood and lavage of ozone+HDM animals. Overall volume of CD25+ cells within airway mucosa increased with HDM exposure. Ozone did not have an additive effect on volume of mucosal CD25+ cells in HDM-exposed animals, but did alter the anatomical distribution of this cell type throughout the proximal and distal airways. We conclude that a window of postnatal development is sensitive to air pollutant and allergen exposure, resulting in immunomodulation of peripheral blood and airway lymphocyte frequency and trafficking.

Citing Articles

Organized lymphatic tissue (BALT) in lungs of rhesus monkeys after air pollutant exposure.

Pabst R, Miller L, Schelegle E, Hyde D Anat Rec (Hoboken). 2020; 303(11):2766-2773.

PMID: 32445535 PMC: 8793891. DOI: 10.1002/ar.24456.

Immunologic and Non-Immunologic Mechanisms Leading to Airway Remodeling in Asthma.

Fang L, Sun Q, Roth M Int J Mol Sci. 2020; 21(3).

PMID: 31979396 PMC: 7037330. DOI: 10.3390/ijms21030757.

Early life allergen and air pollutant exposures alter longitudinal blood immune profiles in infant rhesus monkeys.

Crowley C, Fontaine J, Gerriets J, Schelegle E, Hyde D, Miller L Toxicol Appl Pharmacol. 2017; 328:60-69.

PMID: 28529118 PMC: 5535809. DOI: 10.1016/j.taap.2017.05.006.

Early life exposure to allergen and ozone results in altered development in adolescent rhesus macaque lungs.

Herring M, Putney L, St George J, Avdalovic M, Schelegle E, Miller L Toxicol Appl Pharmacol. 2014; 283(1):35-41.

PMID: 25545987 PMC: 5922427. DOI: 10.1016/j.taap.2014.12.006.

Repair of tracheal epithelium by basal cells after chlorine-induced injury.

Musah S, Chen J, Hoyle G Respir Res. 2012; 13:107.

PMID: 23170909 PMC: 3544626. DOI: 10.1186/1465-9921-13-107.

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