Photoconversion in Orange and Red Fluorescent Proteins

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2009 Apr 14

PMID 19363494

Citations 78

Authors

Gert-Jan Kremers

Kristin L Hazelwood

Christopher S Murphy

Michael W Davidson

David W Piston

Affiliations

Soon will be listed here.

Abstract

We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date.

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