Blue-conversion of Organic Dyes Produces Artifacts in Multicolor Fluorescence Imaging

Overview

Journal Chem Sci

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2021 Jul 14

PMID 34257864

Citations 2

Authors

Do-Hyeon Kim

Yeonho Chang

Soyeon Park

Min Gyu Jeong

Yonghoon Kwon

Kai Zhou

Jungeun Noh

Yun-Kyu Choi

Triet Minh Hong

Young-Tae Chang

Sung Ho Ryu

Affiliations

Soon will be listed here.

Abstract

Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by invading predefined spectral windows, as demonstrated in the case study using EGFR and Tensin2. These multicolor image artifacts become much critical in localization-based superresolution microscopy as the blue-converted dyes are photoactivatable. We provide a practical guideline for the use of organic dyes for multicolor imaging to prevent artifacts derived by blue-conversion.

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