Bright Monomeric Red Fluorescent Protein with an Extended Fluorescence Lifetime

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2007 Jun 19

PMID 17572680

Citations 319

Authors

Ekaterina M Merzlyak

Joachim Goedhart

Dmitry Shcherbo

Mariya E Bulina

Aleksandr S Shcheglov

Arkady F Fradkov

Anna Gaintzeva

Konstantin A Lukyanov

Sergey Lukyanov

Theodorus W J Gadella

Dmitriy M Chudakov

Affiliations

Soon will be listed here.

Abstract

Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.

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