Embryonic Stem Cell-specific MicroRNAs Promote Induced Pluripotency

Overview

Journal Nat Biotechnol

Specialty Biotechnology

Date 2009 Apr 14

PMID 19363475

Citations 340

Authors

Robert L Judson

Joshua E Babiarz

Monica Venere

Robert Blelloch

Affiliations

Soon will be listed here.

Abstract

This report demonstrates that introduction of microRNAs (miRNAs) specific to embryonic stem cells enhances the production of mouse induced pluripotent stem (iPS) cells. The miRNAs miR-291-3p, miR-294 and miR-295 increase the efficiency of reprogramming by Oct4, Sox2 and Klf4, but not by these factors plus cMyc. cMyc binds the promoter of the miRNAs, suggesting that they are downstream effectors of cMyc during reprogramming. However, unlike cMyc, the miRNAs induce a homogeneous population of iPS cell colonies.

Citing Articles

The miR-290 and miR-302 clusters are essential for reprogramming of fibroblasts to induced pluripotent stem cells.

Ye J, Boileau R, Parchem R, Judson-Torres R, Blelloch R Stem Cells. 2025; 43(2).

PMID: 40037390 PMC: 11879289. DOI: 10.1093/stmcls/sxae080.

Analysis of a mouse germ cell tumor model establishes pluripotency-associated miRNAs as conserved serum biomarkers for germ cell cancer detection.

Loehr A, Timmerman D, Liu M, Gillis A, Matthews M, Bloom J Sci Rep. 2025; 15(1):4452.

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The maternal-to-zygotic transition: reprogramming of the cytoplasm and nucleus.

Kojima M, Hoppe C, Giraldez A Nat Rev Genet. 2024; .

PMID: 39587307 DOI: 10.1038/s41576-024-00792-0.

Advances in understanding the regulation of pluripotency fate transition in embryonic stem cells.

Jia Y, Yu Y, Guan L Front Cell Dev Biol. 2024; 12:1494398.

PMID: 39479513 PMC: 11521825. DOI: 10.3389/fcell.2024.1494398.

The miR-290 and miR-302 clusters are essential for reprogramming of fibroblasts to induced pluripotent stem cells.

Ye J, Boileau R, Parchem R, Judson-Torres R, Blelloch R bioRxiv. 2024; .

PMID: 39282363 PMC: 11398367. DOI: 10.1101/2024.09.02.610895.

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