Characterization of a Novel Ghrelin Cell Reporter Mouse

Overview

Journal Regul Pept

Specialty Biochemistry

Date 2009 Apr 14

PMID 19361544

Citations 49

Authors

Ichiro Sakata

Yoshihide Nakano

Sherri Osborne-Lawrence

Sherry A Rovinsky

Charlotte E Lee

Mario Perello

Jason G Anderson

Roberto Coppari

Guanghua Xiao

Bradford B Lowell

Joel K Elmquist

Jeffrey M Zigman

Affiliations

Soon will be listed here.

Abstract

Ghrelin is a hormone that influences many physiological processes and behaviors, such as food intake, insulin and growth hormone release, and a coordinated response to chronic stress. However, little is known about the molecular pathways governing ghrelin release and ghrelin cell function. To better study ghrelin cell physiology, we have generated several transgenic mouse lines expressing humanized Renilla reniformis green fluorescent protein (hrGFP) under the control of the mouse ghrelin promoter. hrGFP expression was especially abundant in the gastric oxyntic mucosa, in a pattern mirroring that of ghrelin immunoreactivity and ghrelin mRNA. hrGFP expression also was observed in the duodenum, but not in the brain, pancreatic islet, or testis. In addition, we used fluorescent activated cell sorting (FACS) to collect and partially characterize highly enriched populations of gastric ghrelin cells. We suggest that these novel ghrelin-hrGFP transgenic mice will serve as useful tools to better understand ghrelin cell physiology.

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